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首页> 外文期刊>Journal of Clinical Microbiology >Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens
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Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens

机译：替代核酸提取方案在ProGastro SSCS分析中检测细菌性肠病原的应用

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As an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. ProGastro SSC and Shiga toxin-producing Escherichia coli (i.e., STEC) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. Following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column methods. Mean processing times for manual and column methods were 30.5 and 69.2 min, respectively. Concordance of investigational methods with automated extraction was ≥98.8%.
机译：作为自动提取的替代方法，通过研究性裂解/加热（即，手动）和色谱/离心（即，柱）方法对粪便样本进行处理。 ProGastro SSC和产生志贺毒素的大肠埃希氏菌（即STEC）的不确定度为自动化样品的1.0％至3.0％，手动样品的11.9％和柱方法的24.8％至37.6％。对247个样品进行冻融后，手动方法的不确定度为1.6％至2.4％，色谱柱方法的不确定度为0.8至5.3％。手动和色谱柱方法的平均处理时间分别为30.5和69.2分钟。研究方法与自动提取的一致性≥98.8％。

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