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首页> 外文期刊>Journal of Clinical Microbiology >Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales
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Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales

机译：对人畜共患病病原体进行病理分型

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Streptococcus suis is one of the most important zoonotic bacterial pathogens of pigs, causing significant economic losses to the global swine industry. S. suis is also a very successful colonizer of mucosal surfaces, and commensal strains can be found in almost all pig populations worldwide, making detection of the S. suis species in asymptomatic carrier herds of little practical value in predicting the likelihood of future clinical relevance. ABSTRACT Streptococcus suis is one of the most important zoonotic bacterial pathogens of pigs, causing significant economic losses to the global swine industry. S. suis is also a very successful colonizer of mucosal surfaces, and commensal strains can be found in almost all pig populations worldwide, making detection of the S. suis species in asymptomatic carrier herds of little practical value in predicting the likelihood of future clinical relevance. The value of future molecular tools for surveillance and preventative health management lies in the detection of strains that genetically have increased potential to cause disease in presently healthy animals. Here we describe the use of genome-wide association studies to identify genetic markers associated with the observed clinical phenotypes (i) invasive disease and (ii) asymptomatic carriage on the palatine tonsils of pigs on UK farms. Subsequently, we designed a multiplex PCR to target three genetic markers that differentiated 115 S. suis isolates into disease-associated and non-disease-associated groups, that performed with a sensitivity of 0.91, a specificity of 0.79, a negative predictive value of 0.91, and a positive predictive value of 0.79 in comparison to observed clinical phenotypes. We describe evaluation of our pathotyping tool, using an out-of-sample collection of 50 previously uncharacterized S. suis isolates, in comparison to existing methods used to characterize and subtype S. suis isolates. In doing so, we show our pathotyping approach to be a competitive method to characterize S. suis isolates recovered from pigs on UK farms and one that can easily be updated to incorporate global strain collections.
机译：猪链球菌是猪最重要的人畜共患细菌病原体之一，给全球养猪业造成重大经济损失。猪链球菌也是非常成功的粘膜表面定居者，在全世界几乎所有猪群中都发现了共生菌株，这使得在无症状携带者中检测到猪链球菌的种种，在预测未来临床相关性方面没有实际价值。 。摘要猪链球菌是猪最重要的人畜共患细菌病原体之一，给全球养猪业造成重大经济损失。猪链球菌也是非常成功的粘膜表面定居者，在全世界几乎所有猪群中都发现了共生菌株，这使得在无症状携带者中检测到猪链球菌的种种，在预测未来临床相关性方面没有实际价值。 。未来用于监视和预防性健康管理的分子工具的价值在于，检测出在遗传上具有增加的能力在目前健康的动物中致病的菌株。在这里，我们描述了使用全基因组关联研究来确定与观察到的临床表型相关的遗传标记（i）侵入性疾病和（ii）在英国农场的猪的tons扁桃体上无症状携带。随后，我们设计了一种多重PCR，以靶向三种遗传标记，这些遗传标记将115株猪链球菌分离为疾病相关组和非疾病相关组，其灵敏度为0.91，特异性为0.79，阴性预测值为0.91与观察到的临床表型相比，阳性预测值为0.79。与用于表征和亚型猪链球菌的现有方法相比，我们使用50个先前未鉴定的猪链球菌分离物的样本外收集描述了我们的病理分型工具的评估。通过这样做，我们证明了我们的病理分型方法是表征英国农场从猪中回收的猪链球菌分离物的一种竞争性方法，并且可以轻松进行更新以纳入全球菌株收集。

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