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首页> 外文期刊>Journal of cell biology >Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis
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Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis

机译：核仁中的信号识别颗粒RNA定位不同于核糖体合成的经典位点

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摘要

The nucleolus is the site of ribosome biosynthesis, but is now known to have other functions as well. In the present study we have investigated how the distribution of signal recognition particle (SRP) RNA within the nucleolus relates to the known sites of ribosomal RNA synthesis, processing, and nascent ribosome assembly (i.e., the fibrillar centers, the dense fibrillar component (DFC), and the granular component). Very little SRP RNA was detected in fibrillar centers or the DFC of the nucleolus, as defined by the RNA polymerase I–specific upstream binding factor and the protein fibrillarin, respectively. Some SRP RNA was present in the granular component, as marked by the protein B23, indicating a possible interaction with ribosomal subunits at a later stage of maturation. However, a substantial portion of SRP RNA was also detected in regions of the nucleolus where neither B23, UBF, or fibrillarin were concentrated. Dual probe in situ hybridization experiments confirmed that a significant fraction of nucleolar SRP RNA was not spatially coincident with 28S ribosomal RNA. These results demonstrate that SRP RNA concentrates in an intranucleolar location other than the classical stations of ribosome biosynthesis, suggesting that there may be nucleolar regions that are specialized for other functions.
机译：核仁是核糖体生物合成的位点，但现在也知道它还具有其他功能。在本研究中，我们研究了核仁中信号识别颗粒（SRP）RNA的分布如何与核糖体RNA合成，加工和新生核糖体组装的已知位点（即，纤维状中心，致密纤维状成分（DFC） ），以及颗粒状成分）。分别由RNA聚合酶I特异的上游结合因子和原纤维蛋白定义，在原纤维中心或核仁DFC中仅检测到很少的SRP RNA。如蛋白B23所示，一些SRP RNA存在于颗粒成分中，表明在成熟的后期可能与核糖体亚基相互作用。但是，在B23，UBF或原纤维蛋白均未浓缩的核仁区域中也检测到了大部分SRP RNA。双探针原位杂交实验证实核仁SRP RNA的很大一部分与28S核糖体RNA在空间上不重合。这些结果表明，SRP RNA集中在核糖体生物合成的经典工位以外的核仁内位置，这表明可能存在专门用于其他功能的核仁区域。

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