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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Detection of FUR1 Gene in 5-Flucytosine Resistant Candida Isolates in Vaginal Candidiasis Patients
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Detection of FUR1 Gene in 5-Flucytosine Resistant Candida Isolates in Vaginal Candidiasis Patients

机译：阴道念珠菌病患者5-氟胞嘧啶抗性念珠菌分离株中FUR1基因的检测

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Background and Aims: This study was done to detect the prevalence, risk factors for vaginal candidiasis in Chennai and to evaluate different methods for speciation of Candida isolates from vaginal candidiasis patients. This study was also aimed at detecting resistance patterns of Candida spp to common antifungals and at detecting mutant FUR1 genes in 5-Flucytosine (5 FC) resistant isolates. Material and Methods: Two hundred clinically suspected vaginal candidiasis patients were screened for candidiasis and isolated Candida were speciated by standard morphological and biochemical tests (sugar fermentation and assimilation) and by using CHROM agar-Candida medium. Antifungal susceptibility was performed by disk diffusion method (CLSI M44-A) using fluconazole, itraconazole and 5FC disks. Five FC resistant isolates were subjected to PCR for detection of mutant FUR1 genes.Results: A total of 72 (36%) Candida spp. were obtained. Vaginal candidiasis was more prevalent in 31-40 years age group and among those with poor genital hygiene and who wore tight fitting syntheticylon underclothes . C.albicans (35), C.tropicalis (8), C.glabrata (21), C.krusei (4) were identified by both carbohydrate assimilation test and by using CHROM agar-Candida medium. C.kefyr (2) and C.parapsilosis (2) could not be identified using CHROM agar-Candida. Resistance to fluconazole, itraconazole and 5-flucytosine was seen in 19.44%, 23.61% and 41.66% of the isolates respectively. Mutant FUR1 gene was detected in all the Candida spp that were resistant to 5FC. Conclusion: C.albicans was the commonest species which caused vaginal candidiasis in Chennai. Though CHROM agar-candida medium is a useful differential isolation medium capable of early presumptive identification of Candida species, it could not identify C.kefyr and C.parapsilosis. Azole resistance was low in C. albicans but it was high in non-albicans Candida spp. Prevalence of primary resistance to 5-flucytosine was high in the strains studied and in all of them, it was mediated by mutant FUR1 gene.
机译：背景与目的：本研究旨在检测钦奈地区阴道念珠菌病的患病率和危险因素，并评估从阴道念珠菌病患者中分离出念珠菌的不同方法。这项研究还旨在检测假丝酵母对常见抗真菌药的耐药模式，并检测5-氟胞嘧啶（5 FC）耐药菌株中的突变FUR1基因。材料和方法：对200名临床疑似阴道念珠菌病患者进行了念珠菌病筛查，并通过标准形态学和生化测试（糖发酵和同化作用）并使用CHROM琼脂-Candida培养基对分离的念珠菌进行了鉴定。使用氟康唑，伊曲康唑和5FC圆盘，通过圆盘扩散法（CLSI M44-A）进行抗真菌药敏性。对5株对FC耐药的菌株进行PCR检测突变FUR1基因。结果：共有72个（36％）念珠菌。获得了。阴道念珠菌病在31-40岁年龄组以及生殖器卫生较差且穿着紧身合成/尼龙内衣的人群中更为普遍。通过碳水化合物同化试验和使用CHROM琼脂-Candida培养基鉴定了白色念珠菌（35），热带念珠菌（8），光滑念珠菌（21），克鲁氏梭菌（4）。使用CHROM琼脂-Candida无法鉴定C.kefyr（2）和C.parapsilosis（2）。分离株对氟康唑，伊曲康唑和5-氟胞嘧啶的耐药性分别为19.44％，23.61％和41.66％。在所有对5FC有抗性的念珠菌中检测到突变的FUR1基因。结论：白色念珠菌是引起钦奈阴道念珠菌病的最常见物种。尽管CHROM琼脂念珠菌培养基是一种有用的差异分离培养基，能够早期推测假丝酵母菌种，但它不能鉴别克菲氏假丝酵母和假丝酵母。白色念珠菌的耐唑性较低，但非白色念珠菌的耐偶氮性较高。在所研究的菌株中，对5-氟胞嘧啶的原发耐药率很高，在所有菌株中，都是由突变的FUR1基因介导的。

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