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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Identification and Differentiation of Carbapenemases in Klebsiella Pneumoniae: A Phenotypic Test Evaluation Study from Jaipur, India
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Identification and Differentiation of Carbapenemases in Klebsiella Pneumoniae: A Phenotypic Test Evaluation Study from Jaipur, India

机译：肺炎克雷伯菌中碳青霉烯酶的鉴定和分化：来自印度斋浦尔的表型测试评价研究

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Background: Carbapenem resistance is one of the major threats faced in antimicrobial treatment of infections caused by gram negative organisms. In recent years, carbapenem resistance has emerged in Klebsiella pneumoniae isolates due to acquisition of carbapenemases which belong to Ambler class A KPC type enzymes or to Ambler class B metallo- ? -lactamases (MBL). Routine lab detection of carbapenemase producing K. pneumoniae isolates is crucial, both for a therapeutic management and an efficient infection control.Materials and Methods: A study was conducted on 60 carbapenem resistant Klebsiella pneumoniae strains which were isolated from various clinical samples over a period of one year (September 2010-August 2011), at a tertiary care hospital in Jaipur. Phenotypic confirmatory test was done by using discs of Meropenem alone and those with phenyl boronic acid (PBA) or Ethylenediaminetetraacetic acid (EDTA) or both, for detection of carbapenemase production and differentiation of KPC and MBL enzymes.Results: Of the 60 carbapenem resistant Klebsiella pneumoniae isolates, 53 (88.33%) were found to be MBL producers, 4(6.66%) were found to be MBL and KPC co-producers and the rest of the 3(5%) isolates were negative for both MBL and KPC production, as was seen by combined disc testing.Conclusion: The combined disc test is a simple test which can be used for differentiation of carbapenemases and it can be easily incorporated in routine microbiology lab testing.
机译：背景：对碳青霉烯类药物的耐药性是革兰氏阴性菌引起的感染的抗菌治疗面临的主要威胁之一。近年来，由于获得属于Ambler A类KPC型酶或Ambler B类金属酶的碳青霉烯酶，肺炎克雷伯菌分离物中出现了碳青霉烯抗性。 -内酰胺酶（MBL）。常规实验室检测产生碳青霉烯酶的肺炎克雷伯菌分离株，对于治疗管理和有效的感染控制都至关重要。材料与方法：研究了60株耐碳青霉烯酶的肺炎克雷伯菌菌株，这些菌株是在一段时间内从各种临床样品中分离出来的。一年（2010年9月至2011年8月）在斋浦尔的一家三级护理医院。通过单独使用美洛培南的碟片和含苯硼酸（PBA）或乙二胺四乙酸（EDTA）或两者的碟片进行表型确认试验，以检测碳青霉烯酶的产生以及KPC和MBL酶的分化。发现肺炎支原体分离物为MBL生产者的有53（88.33％），是MBL和KPC共同生产者的4（6.66％），其余3（5％）分离物对MBL和KPC的生产均呈阴性，结论：联合椎间盘检查是一种简单的测试，可用于区分碳青霉烯酶，并且可以轻松地纳入常规微生物实验室测试中。

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