...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical and Diagnostic Research >Detection of NDM-1 in Clinical Isolates of Klebsiella Pneumoniae from Northeast India
【24h】

Detection of NDM-1 in Clinical Isolates of Klebsiella Pneumoniae from Northeast India

机译：印度东北部肺炎克雷伯菌临床分离株中NDM-1的检测

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction: The growing incidence of the resistance to carbapenems among the members of Enterobacteriaceae is of major concern throughout the world, as these drugs are often used now-a-days as the last line of effective treatment against the serious infections which are caused by multidrug resistant Enterobacteriaceae. We detected blaNDM-1 in the Klebsiella pneumoniae isolates which were collected from a tertiary care referral hospital in northeast India.Materials and Methods: A total of 219 non-duplicated K. pneumoniae isolates were recovered from various clinical samples between August 2009 and July 2010. These isolates were screened for the detection of carbapenemase production on the basis of their reduced susceptibility to meropenem or ertapenem by the disc diffusion method. The screened isolates were further phenotypically studied for carbapenemase production by the modified Hodge test and the detection of metallo-β-lactamase production was carried out by using the combined disc test. All the screened isolates were also subjected to PCR detection of the blaNDM-1 gene and the additional bla genes which coded for TEM, SHV, CTX-M, and AmpC.Results: Out of the 219 isolates, 19 were screened for the detection of carbapenemase production on the basis of their reduced susceptibility to meropenem or ertapenem. Both the phenotypic confirmatory tests showed positive results for all the screening positive isolates, although few weakly positive reactions for the modified Hodge test were observed. All the screened isolates were found to be positive for blaNDM-1. Each of the blaNDM-1 possessing isolates was also found to be positive for one or more additional bla genes.Conclusion: This study indicated that the incidence of blaNDM-1, together with that of one or more additional bla genes among the K. pneumoniae isolates from hospitalized and non hospitalized patients in northeast India.
机译：简介：肠杆菌科成员对碳青霉烯类药物的耐药性越来越高，这在世界范围内引起了广泛关注，因为这些药物如今经常被用作对抗多种药物引起的严重感染的有效方法的最后一道耐药的肠杆菌科。我们在印度东北三级转诊医院收集的肺炎克雷伯菌分离株中检测到blaNDM-1。材料与方法：从2009年8月至2010年7月，从各种临床样本中共回收了219株非重复的肺炎克雷伯菌。根据这些分离物通过圆盘扩散法降低了对美洛培南或厄他培南的敏感性，筛选出用于检测碳青霉烯酶生产的菌株。通过改良的Hodge试验对筛选出的分离物进一步表型研究碳青霉烯酶的产生，并通过组合圆盘试验对金属β-内酰胺酶的产生进行检测。所有筛选出的分离株还接受了blaNDM-1基因和其他TEM，SHV，CTX-M和AmpC编码的bla基因的PCR检测。结果：从219株分离株中，筛选出19株用于检测HBV。碳青霉烯酶的生产是基于它们对美洛培南或厄他培南的敏感性降低。两种表型确证测试均显示所有筛选阳性分离株均呈阳性结果，尽管改良的Hodge检验几乎观察不到弱阳性反应。发现所有筛选的分离株均对blaNDM-1呈阳性。结论：这项研究表明，肺炎克雷伯氏菌中blaNDM-1的发生率以及一个或多个其他bla基因的发生率也很高。来自印度东北部住院和非住院患者的分离株。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号