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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women
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Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women

机译：培养，抗原检测和聚合酶链反应检测孕妇B群链球菌（GBS）阴道定植的评价。

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Background and Objective: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women.Settings and Design: Observational descriptive study was done in a tertiary care hospital in Southern India.Materials and Methods: VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5% sheep blood agar, Conventional PCR assay and antigen detection method were performed.The performance of antigen detection and PCR methods were compared with culture. Statistical analysis: Statistical analysis was performed by Chi-Square test.Results: GBS cultures were positive for 16% of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100%, 92.86%, 72.73%, 100% and similarly for that of PCR was 100%, 45.23%, 25.80%, 100%, respectively.Conclusion: Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.
机译：背景与目的：B组链球菌感染是新生儿发病和死亡的重要原因。 B组链球菌（GBS）的围产期阴道-直肠（VR）转运的早期检测在新生儿感染的管理中很重要。该研究的目的是评估培养，抗原检测和聚合酶链反应在孕妇中检测GBS的情况。设置与设计：在印度南部的一家三级医院进行观察性描述性研究。材料与方法：收集VR拭子在妊娠35至37周时从50名妇女中分离出来。在选择性的Lim富集肉汤中培养，然后在5％羊血琼脂上培养，进行常规PCR测定和抗原检测方法。将抗原检测和PCR方法的性能与培养物进行比较。统计分析：通过卡方检验进行统计分析。结果：GBS培养阳性率为16％（50个样本中有8个）。将培养作为金标准，抗原检测的灵敏度，特异性，阳性预测值和阴性预测值分别为100％，92.86％，72.73％，100％，而PCR的相似度分别为100％，45.23％，25.80％，100结论：抗原检测方法是一种快速，灵敏和特异的检测孕期GBS定植者的方法。

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