...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical and Diagnostic Research >Erythromycin Resistance Determinants in Clinical Gram Positive Cocci Isolated from Nigerian Patients
【24h】

Erythromycin Resistance Determinants in Clinical Gram Positive Cocci Isolated from Nigerian Patients

机译：尼日利亚患者临床革兰氏阳性球菌中的红霉素耐药性决定因素

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The emergence of erythromycin resistant Gram positive cocci in various parts of the world has become worrisome in clinical settings in recent times, however there is little or no information on the determinants of erythromycin resistance from Nigeria.Aim: This study investigated the determinants of erythromycin resistance in clinical Gram positive cocci bacteria isolated from Nigeria patients.Materials and Methods: Assembly of isolates of non-duplicate staphylococci from various clinical specimens from south western and northern hospitals of Nigeria were collected. While enterococci were obtained from our culture collection which were previously collected from healthy people from community. Characterisation of 25 staphylococci and enterococci each were done using standard microbiology procedures, susceptibility pattern to erythromycin and other panel of antimicrobial agents including Minimum Inhibitory Concentrations (MICs) to erythromycin was determined. Erythromycin resistance genes were amplified using Polymerase Chain Reaction (PCR).Results: None of the strains had ermA and mefA but the strains showed heterogeneous possession of ermB, ermC, msrA and msrB genes in no particular pattern including multiple genes acquisition. MIC50 and MIC90 of staphylococci strains to erythromycin were 2 μg/mL and >64 μg/mL, respectively; wherein 7 (28%) were sensitive to erythromycin, while 11 (44%) of enterococci were sensitive to erythromycin with MIC50 and MIC90 of 1 and >64 μg/mL, respectively. One of the staphylococci isolates had inducible clindamycin resistance.Conclusion: In conclusion, high level staphylococci and enterococci resistance was found to various antibiotics with limited therapeutic option. Ribosomal methylation and efflux are the main resistant determinants found in these isolates.
机译：近年来，在世界各地，红霉素耐药革兰氏阳性球菌在临床上的出现令人担忧，但是来自尼日利亚的红霉素耐药性决定因素的信息很少甚至没有。目的：本研究调查了这些因素材料和方法：从尼日利亚西南和北部医院的各种临床标本中收集非重复葡萄球菌的分离株。肠球菌是从我们的文化馆藏中获得的，而这些文化馆藏以前是从社区的健康人那里收集的。使用标准的微生物学程序分别对25种葡萄球菌和肠球菌进行鉴定，确定对红霉素的敏感性模式，并确定其他一系列抗菌药物（包括对红霉素的最低抑菌浓度（MIC））。结果：所有菌株均没有ermA和mefA，但菌株均具有ermB，ermC，msrA和msrB基因异质性，没有特定的模式，包括多基因采集。葡萄球菌菌株对红霉素的MIC50和MIC90分别为2μg/ mL和> 64μg/ mL;其中7（28％）对红霉素敏感，而11（44％）肠球菌对红霉素敏感，MIC50和MIC90分别为1和> 64μg/ mL。葡萄球菌分离株之一具有可诱导的克林霉素耐药性。结论：总结而言，发现对多种抗生素的高水平葡萄球菌和肠球菌耐药性具有有限的治疗选择。核糖体甲基化和外排是在这些分离物中发现的主要抗性决定簇。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号