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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Rapid Screening for Carbapenem Resistant Organisms: Current Results andFuture Approaches
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Rapid Screening for Carbapenem Resistant Organisms: Current Results andFuture Approaches

机译：快速筛选抗碳青霉烯的生物：当前结果和未来方法

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Carbapenem producing Enterobacteriaceae (CPE) is a major public health threat. A total of 120 carbapenem resistant E.coli (n=32) and K.pneumoniae (n=88) from blood stream infections were screened for the presence of carbapenem resistant genes KPC, NDM, IMP, VIM, and OXA-48 like using both conventional multiplex PCR and Xpert? Carba-R test. Additionally 26 faeces samples were directly screened with Xpert? Carba-R test. Of the tested isolates, 40% (n=48) of NDM and 39.2% (n=47/) of OXA-48-like were identified. Co-production of OXA-48 and NDM was seen in 15 (12.5%) isolates. In Xpert? Carba-R test, only NDM was identified in 55% (n=66) of tested isolates. Of the tested faeces samples, 12 were identified as carbapenemase producers: nine with NDM, two with the co-production of NDM and VIM and in Klebsiella spp (n=1), NDM and KPC co-production was seen. However, Xpert? Carba-R test fails to detect OXA-48 like as compared with multiplex PCR. The sensitivity, specificity, PPV, NPV of Xpert? Carba-R test was 100%, 77%, 96% and 100% respectively. Incorporation of OXA-48 like specific sequence in the panel of Xpert? Carba-R test may improve its sensitivity and maximize the coverage of assay.
机译：产生碳青霉烯的肠杆菌科（CPE）是主要的公共卫生威胁。筛选了120例来自血流感染的耐碳青霉烯的大肠杆菌（n = 32）和肺炎克雷伯菌（n = 88），例如使用以下方法检测是否存在耐碳青霉烯的基因KPC，NDM，IMP，VIM和OXA-48常规多重PCR和Xpert？ Carba-R测试。另外，直接用Xpert？筛选了26个粪便样品。 Carba-R测试。在测试的分离物中，鉴定出40％（n = 48）的NDM和39.2％（n = 47 /）的OXA-48-like。在15个（12.5％）分离物中发现了OXA-48和NDM的联合生产。在Xpert中？ Carba-R测试中，在55％（n = 66）的分离株中仅鉴定出NDM。在测试的粪便样本中，有12个被确定为碳青霉烯酶生产者：9个为NDM，两个为NDM和VIM的共同生产，而在克雷伯菌属（n = 1）中，可以看到NDM和KPC的共同生产。但是，Xpert？与多重PCR相比，Carba-R测试无法检测OXA-48。 Xpert的敏感性，特异性，PPV，NPV？ Carba-R测试分别为100％，77％，96％和100％。在XpertTM面板中掺入类似OXA-48的特定序列。 Carba-R检测可提高其灵敏度并最大程度地扩大检测范围。

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