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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Molecular Strain Typing of Clinical Isolates, Trichophyton rubrum using Non Transcribed Spacer (NTS) Region as a Molecular Marker DC04-DC09
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Molecular Strain Typing of Clinical Isolates, Trichophyton rubrum using Non Transcribed Spacer (NTS) Region as a Molecular Marker DC04-DC09

机译：使用非转录间隔区（NTS）区域作为分子标记，对临床分离株红毛癣菌的分子菌株进行分型DC04-DC09

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Introduction: Dermatophytes are a group of fungi which infect keratinized tissues and causes superficial mycoses in humans and animals. The group comprises of three major genera, Trichophyton, Microsporum and Epidermophyton. Among them Trichophyton rubrum is a predominant anthrophophilic fungi which causes chronic infections. Although, the infection is superficial and treatable, reinfection/coinfection causes inflation in the treatment cost. Identifying the source and mode of transmission is essential to prevent its transmission. Accurate discrimination is required to understand the clinical (relapse or reinfection) and epidemiological implications of the genetic heterogeneity of this species. Polymorphism in the Non Transcribed Spacer (NTS) region of ribosomal DNA (rDNA) clusters renders an effective way to discriminate strains among T. rubrum.Aim: To carry out the strain typing of the clinical isolates, Trichophyton rubrum using NTS as a molecular marker.Materials and Methods: Seventy T. rubrum clinical isolates obtained from April-2011-March 2013, from Sri Ramachandra Medical Centre, Chennai, Tamil Nadu, India, were identified by conventional phenotypic methods and included in this prospective study. The isolates were then subjected to Polymerase Chain Reaction (PCR) targeting two subrepeat elements (SREs), TRS-1 and TRS-2 of the NTS region.Results: Strain-specific polymorphism was observed in both subrepeat loci. Total, nine different strains were obtained on combining both TRS-1 and TRS-2, SREs.Conclusion: The outcome has given a strong representation for using NTS region amplification in discriminating the T. rubrum clinical isolates. The method can be adapted as a tool for conducting epidemiology and population based study in T. rubrum infections. This will help in future exploration of the epidemiology of T. rubrum.
机译：简介：皮肤癣菌是一组真菌，它们感染角化组织并在人和动物中引起浅表霉菌病。该组包括三个主要属，毛癣菌，小孢子菌和表皮癣菌。其中红毛癣菌是一种主要的嗜盐真菌，会引起慢性感染。尽管感染是表面的并且可以治疗的，但是再感染/共感染会导致治疗费用的上涨。确定传播的来源和方式对于防止其传播至关重要。需要准确的区分才能了解该物种遗传异质性的临床（复发或再感染）和流行病学意义。核糖体DNA（rDNA）簇的非转录间隔区（NTS）区域的多态性为区分红毛病菌提供了一种有效的方法。材料与方法：通过常规表型方法鉴定了从印度泰米尔纳德邦钦奈的Sri Ramachandra Medical Center于2011年4月至2011年3月获得的70株红毛锥虫临床分离株，并将其包括在该前瞻性研究中。然后对分离物进行针对两个亚重复元件（SRE），NTS区域的TRS-1和TRS-2的聚合酶链反应（PCR）。结果：在两个亚重复基因座中均观察到了菌株特异性多态性。结合TRS-1和TRS-2，SREs共获得了九种不同的菌株。结论：该结果有力地证明了使用NTS区域扩增技术来鉴别红曲霉临床分离株。该方法可以用作进行红斑锥虫感染的流行病学和基于人群的研究的工具。这将有助于将来探索红毛锥虫的流行病学。

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