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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Diagnostic Utility of Epithelial Membrane Antigen (EMA) and Calretinin (CAL) in Effusion Cytology EC36-EC39
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Diagnostic Utility of Epithelial Membrane Antigen (EMA) and Calretinin (CAL) in Effusion Cytology EC36-EC39

机译：上皮细胞膜抗原（EMA）和降钙素（CAL）在积液细胞学EC36-EC39中的诊断效用

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Introduction: Fluid cytology and subsequent utilization of immunocytology on cell block is being used commonly for increasing the diagnostic accuracy in effusion cytology.Aim: To do cytological analysis of fluids and evaluate the role of Epithelial Membrane Antigen (EMA) and Calretinin (CAL) to differentiate between reactive and malignant cell in pleural and peritoneal fluids.Materials and Methods: This was a prospective study carried out in Department of Pathology, Shri Guru Ram Rai Institue of Medical and Health Science, for a period of 18 months from January 2014 to June 2015. A total of 253 samples of pleural and peritoneal fluid were studied by May-Gruenwald-Giemsa (MGG) stain and Papanicolaou (Pap) staining. In 73 cases which were suspicious for malignancy, cell blocks were prepared and IHC was done using two immunomarkers- EMA and CAL.Results: A total of 253 cases of effusion cytology were studied out of which 73 were found positive for malignant cells. Maximum cases of malignant cells or atypical cells were seen in peritoneal fluid. A total of 34 cases were histologically positive for malignancy. All these cases showed strong membranous and cytoplasmic positivity for EMA. Most of cases also showed 2%-5% positivity for CAL. There were 38 cases categorized as atypical or reactive mesothelial cell hyperplasia. These cases showed nuclear and cytoplasmic staining for calretinin and none of these were positive for EMA, although 5 cases showed score 2-3, which was considered as negative.Conclusion: The distinction between reactive and malignant mesothelial cells in cytological specimens can be problematic. A combination of CAL and EMA may help in accurate diagnosis.
机译：简介：液体细胞学以及随后在细胞块上使用免疫细胞学通常用于提高积液细胞学的诊断准确性。目的：对液体进行细胞学分析，并评估上皮膜抗原（EMA）和降钙素（CAL）的作用材料和方法：这是一项前瞻性研究，从2014年1月至6月，为期18个月，在Shri Guru Ram Rai医学与健康科学研究院病理学系进行，为期18个月。 2015年。通过May-Gruenwald-Giemsa（MGG）染色和Papanicolaou（Pap）染色研究了总共253例胸膜和腹膜液样品。在73例可疑恶性肿瘤中，准备了细胞块，并使用EMA和CAL这两种免疫标记物进行了IHC。结果：共研究了253例积液细胞学病例，其中73例恶性细胞阳性。在腹膜液中最多见到恶性细胞或非典型细胞。共有34例恶性组织学阳性。所有这些病例均显示出针对EMA的强烈的膜和细胞质阳性。大多数病例还显示CAL阳性率为2％-5％。有38例分类为非典型或反应性间皮细胞增生。这些病例显示了钙网蛋白的核和细胞质染色，但均未显示EMA阳性，尽管5例显示2-3分，被认为是阴性。结论：细胞学标本中反应性和恶性间皮细胞的区分可能存在问题。 CAL和EMA的组合可能有助于准确诊断。

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