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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Assessment of Enamel Surface Microhardness with Different Fluorinated Compounds under pH Cycling Conditions: An in Vitro Study
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Assessment of Enamel Surface Microhardness with Different Fluorinated Compounds under pH Cycling Conditions: An in Vitro Study

机译:在pH循环条件下用不同的氟化物评估牙釉质表面显微硬度的体外研究

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Various studies have reported the effect of fluoride on the surface of dental enamel in preventing the development of carious lesions. Research conducted on the effect of fluoride on demineralization and remineralization has reported on the in vitro pH cycling model, in which samples of enamel or dentin were subjected to pH changes, such as those occurring in the oral cavity, to reduce the advancement of the cavitated lesions.Aim: To evaluate the remineralization of dental enamel after the application of three fluorinated compounds commonly used in paediatric dentistry sodium fluoride, acidulated phosphate fluoride, and silver diamine fluoride and the effect on enamel microhardness under pH cycling conditions.Materials and Methods: Sixty impacted 3rd molars, with intact anatomical crowns recently extracted and without repaired structural defects, were sectioned longitudinally in a mesiodistal direction using a diamond disc, obtaining two working surfaces (buccal and lingual). The 120 working surfaces obtained were immersed for 96 hours in a demineralization solution at 37°C in order to demineralize the enamel surface. All of the demineralized molars were then randomly divided into four groups (n=30 each) and their surfaces were treated with Silver diamine fluoride (SDF), Difluoride silane (DSF), and Acidulated phosphate fluoride (APF), and with no treatment undertaken in the Control group (CG). The samples were immersed in alternating solutions for demineralization and remineralization at pH 4.4 and pH 7.0, respectively. The Vickers hardness number (VHN) was determined after 5, 10, and 15 days using a microdurometer with a load of 25 gf for 20s. Data were analysed using the ANOVA statistical test.Results: The microhardness values obtained from the three remineralization groups (SDF, DSF and APF) were obtained, with the mean microhardness after 10 days of treatment higher in the occlusal and cervical areas and statistically significant differences were found (SDF p=0.016, DSF p=0.043 and APF p=0.035). By Day 15, an increase in mean microhardness was found in the SDF and DSF groups at different depths recorded, and statistically significant differences were found.Conclusion: We found a significant difference among the effects of the three fluorinated compounds (SDF, DSF and APF) on the enamel surface. This finding is clinically significant as the use of remineralizing agents such as SDF, DSF and APF increases the microhardness of the enamel surface, suggesting that fluorinated compounds are effective in the reduction and remineralization of incipient carious lesions in dental enamel.
机译:各种研究报告了氟化物在牙釉质表面上的作用,可预防龋齿病变的发展。关于氟化物对脱矿质和再矿化的影响的研究已报告了体外pH循环模型,该模型对牙釉质或牙本质样品进行了pH变化(例如口腔中发生的pH变化),以减少空化的进展。目的:评估在儿科牙科中通常使用的三种氟化化合物氟化钠,酸化的氟化物氟化物和氟化二胺银的应用后,牙釉质的再矿化以及在pH循环条件下对牙釉质显微硬度的影响。 >材料与方法:用金刚石磨盘沿近中方向纵向切开60颗受冲击的第三磨牙,将其最近取出的完整的解剖冠冠修复,并沿近中视方向纵向切开,获得两个工作面(颊侧和舌侧)。将获得的120个工作表面在37℃的去矿物质溶液中浸泡96小时,以使釉质表面矿化。然后将所有脱矿质磨牙随机分为四组(每组n = 30),并用氟化二胺氟化银(SDF),二氟化硅烷(DSF)和酸化氟化磷(APF)对其表面进行处理。在对照组(CG)中。将样品浸入交替的溶液中以分别在pH 4.4和pH 7.0进行脱盐和再矿化。在5天,10天和15天后,使用微硬度计在25 gf的载荷下20秒钟测定维氏硬度值(VHN)。结果使用三个再矿化组(SDF,DSF和APF)获得显微硬度值,治疗10天后,在咬合和宫颈区域的平均显微硬度更高。发现统计学上的显着差异(SDF p = 0.016,DSF p = 0.043和APF p = 0.035)。到第15天,在SDF和DSF组中记录的不同深度处的平均显微硬度增加了,并且在统计学上也发现了显着差异。结论:我们发现三种氟化物(SDF, DSF和APF)。这一发现在临床上具有重要意义,因为使用再矿化剂(例如SDF,DSF和APF)可提高牙釉质表面的显微硬度,这表明氟化物可有效减少和再矿化牙釉质中初期龋齿病变。

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