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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Association of Androgen Receptor (AR) CAG Repeats and Cytochrome P450 3A5*3 (CYP3A5*3) Gene Polymorphisms in South Indian Men with Prostate Cancer
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Association of Androgen Receptor (AR) CAG Repeats and Cytochrome P450 3A5*3 (CYP3A5*3) Gene Polymorphisms in South Indian Men with Prostate Cancer

机译：南印度男性前列腺癌中雄激素受体（AR）CAG重复与细胞色素P450 3A5 * 3（CYP3A5 * 3）基因多态性的关联

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Introduction: Prostate Cancer (PCA) is the second mostcommon cancer diagnosed in men worldwide. ProstateSpecifc Antigen (PSA) is the readily used biomarker for PCAscreening, but lack of specifcity limits its usefulness. Hence,role of Androgen receptor (AR) CAG repeat polymorphismand Cytochrome P450 3A5*3 gene polymorphism involved inthe metabolism of testosterone, growth and differentiation ofprostate gland were evaluated to assess their association withpathogenesis of prostate lesions.Aim: To determine CAG repeats in AR gene and CYP3A5*3gene polymorphism in South Indian men with PCA and BenignProstate Hyperplasia (BPH).Materials and Methods: Genomic DNA was isolated from 312(100 men with PCA, 109 BPH patients and 103 controls). Formalinfxed paraffn embedded tissue/peripheral blood samples usingsalting out method were used. Polymerase Chain Reaction (PCR)procedure was carried out using specifc primers for AR andCYP3A5*3 genes. Bands were visualised by electrophoresis on2% ethidium bromide (EtBr) stained agarose gel. Odds ratios werecalculated using MedCalcR version 18.2.1 and Chi-square analysiswas carried out to determine the association among groups.Results: AR gene with <22 CAG repeats and ‘GG’ genotypeof CYP3A5*3 gene polymorphism were identifed in 61 (61%),PCA patients, 11 (10%) BPH and 13 (13%) control individuals.A statistically signifcant association was observed betweenAR short repeats and CYP3A5*3 ‘GG’ genotype with PCA(p-value <0.05).Conclusion: The results suggest that the best age for PCAscreening is 48 years or above for early detection and preventionof this malignancy. A <22 CAG repeats in AR gene and ‘GG’genotype of CYP3A5*3 (rs#776746) gene can be togetherconsidered as specifc molecular marker for identifying men ata risk of developing PCA.
机译：简介：前列腺癌（PCA）是全球男性中诊断第二多的常见癌症。前列腺特异抗原（PSA）是PCA筛查中易于使用的生物标志物，但缺乏特异性限制了其用途。因此，评估了雄激素受体（CA）重复序列的多态性和细胞色素P450 3A5 * 3基因多态性与睾丸激素代谢，前列腺生长和分化的关系，以评估其与前列腺病变发病机制的关系。目的：确定AR基因中CAG重复序列CYP3A5 * 3基因多态性与南印度人PCA和前列腺增生（BPH）的基因多态性。材料与方法：从312例（100例PCA男性，109例BPH患者和103例对照）中分离基因组DNA。使用盐析法将甲醛固定在石蜡包埋的组织/外周血中。使用针对AR和CYP3A5 * 3基因的特异性引物进行聚合酶链反应（PCR）过程。通过在2％溴化乙锭（EtBr）染色的琼脂糖凝胶上电泳使条带可见。使用MedCalcR版本18.2.1计算赔率，并进行卡方分析以确定各组之间的关联。结果：在61（61％）人群中鉴定出具有<22 CAG重复的AR基因和CYP3A5 * 3基因多态性的'GG'基因型。 ，PCA患者，11个（10％）BPH和13个（13％）对照个体。观察到AR短重复与CYP3A5 * 3'GG'基因型与PCA的统计学显着相关（p值<0.05）。结论：结果建议进行PCA筛查的最佳年龄是48岁或以上，以便及早发现和预防这种恶性肿瘤。可以将AR基因中的<22个CAG重复和CYP3A5 * 3（rs＃776746）基因的“ GG”基因型一起用作鉴定男性罹患PCA风险的特定分子标记。

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