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首页> 外文期刊>Journal of bacteriology >RESPONSE OF ESCHERICHIA COLI AUXOTROPHS TO HEAT AFTER TREATMENT WITH MUTAGENIC ALKYL METHANESULFONATES
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RESPONSE OF ESCHERICHIA COLI AUXOTROPHS TO HEAT AFTER TREATMENT WITH MUTAGENIC ALKYL METHANESULFONATES

机译：突变型烷基甲基苯磺酸处理后大肠埃希氏菌营养缺陷对热的响应

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Strauss, Bernard S. (The University of Chicago, Chicago, Ill.). Response of Escherichia coli auxotrophs to heat after treatment with mutagenic alkyl methanesulfonates. J. Bacteriol. >83:241–249. 1962.—Heating in buffer at 48 C resulted in a large increase in the frequency of revertants obtained after treatment of restingphase cells of Escherichia coli strain WP-2 (a tryptophan-requiring mutant of strain B/r) with ethyl methanesulfonate. This effect was not obtained with the mutagenic homologue, methyl methanesulfonate. Cells treated with ethyl methanesulfonate lost their sensitivity to heat soon after the start of incubation in growth medium; chloramphenicol added to the growth medium preserved the sensitivity. Cells in the exponential growth phase which were treated with ethyl methanesulfonate were rapidly killed by heat treatment. Methyl methanesulfonate was more effective as a killing agent than the ethyl derivative. Heat effects on mutation frequency were obtained with a second tryptophan-requiring mutant of E. coli B/r and with a tryptophan-requiring mutant of E. coli K-12.
机译：Strauss，Bernard S.（芝加哥大学，伊利诺伊州芝加哥）。诱变的烷基甲磺酸盐处理后，大肠杆菌营养缺陷型对热的响应。 J.细菌。 > 83： 241-249。 1962年。在48°C的缓冲液中加热导致处理大肠埃希氏大肠杆菌WP-2菌株（需要色氨酸的B / r突变体）的静止期细胞后获得的回复子频率大大增加。 ）与甲磺酸乙酯。用诱变的同系物甲磺酸甲酯无法获得这种效果。在生长培养基中开始孵育后不久，用甲磺酸乙酯处理的细胞就失去了对热的敏感性。向生长培养基中添加氯霉素可保持敏感性。用甲磺酸乙酯处理过的处于指数生长期的细胞通过热处理迅速被杀死。甲磺酸甲酯比乙基衍生物更有效地用作杀灭剂。用第二个需要色氨酸的E突变体获得了对突变频率的热效应。大肠杆菌 B / r和需要 E的色氨酸突变体。大肠杆菌 K-12。

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