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首页> 外文期刊>Journal of bacteriology >Behavior of Coliphage Lambda in Hybrids Between Escherichia coli and Salmonella
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Behavior of Coliphage Lambda in Hybrids Between Escherichia coli and Salmonella

机译：大肠杆菌和沙门氏菌杂种中Coliphage Lambda的行为

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Salmonella typhosa hybrids able to adsorb lambda were obtained by mating S. typhosa recipients with Escherichia coli K-12 donors. After adsorption of wild-type λ to these S. typhosa hybrids, no plaques or infective centers could be detected. E. coli K-12 gal+ genes carried by the defective phage λdg were transduced to S. typhosa hybrids with HFT lysates derived from E. coli heterogenotes. The lysogenic state which resulted in the S. typhosa hybrids after gal+ transduction differed from that of E. coli. Ability to produce λ, initially present, was permanently segregated by transductants of the S. typhosa hybrid. S. typhosa lysogens did not lyse upon treatment for phage induction with mitomycin C, ultraviolet light, or heat in the case of thermoinducible λ. A further difference in the behavior of λ in Salmonella hybrids was the absence of zygotic induction of the prophage when transferred from E. coli K-12 donors to S. typhosa. A new λ mutant class, capable of forming plaques on S. typhosa hybrids refractory to wild-type λ, was isolated at low frequency by plating λ on S. typhosa hybrid WR4254. Such mutants have been designated as λsx, and a mutant allele of λsx was located between the P and Q genes of the λ chromosome. Plaques were formed also on the S. typhosa hybrid host with a series of λi21 hybrid phages which contain the N gene of phage 21. The significance of these results in terms of Salmonella species as hosts for λ is discussed.
机译：通过使 S交配获得了能够吸附λ的伤寒沙门氏菌杂种。大肠杆菌 K-12供体的伤寒受体。将野生型λ吸附到这些 S之后。斑疹伤寒杂种，没有发现斑块或感染中心。 E。缺陷噬菌体λ dg 携带的大肠杆菌K-12 gal + 基因被转导至 S。 typhosa 杂种与 E衍生的HFT裂解物。大肠菌导致 S的溶原状态。 gal + 转导后的typhosa 杂种与 E不同。大肠杆菌。最初存在的产生λ的能力被 S的转导物永久隔离。 typhosa 杂种。 S。在用丝裂霉素C，紫外线或热诱导的λ诱导的噬菌体诱导处理后，鼠伤寒菌的溶原原没有溶解。沙门氏菌沙门氏菌杂种中λ行为的另一个差异是从 E转移时不存在合子诱导的前噬菌体。大肠杆菌 K-12供体到 S。伤寒。一种新的λ突变体类别，能够在 S上形成噬菌斑。通过将λ镀在 S上以低频分离出耐野生型λ的typhosa 杂种。 typhosa 混合WR4254。这样的突变体被命名为λ sx ，并且λ sx 的突变等位基因位于λ染色体的P和Q基因之间。在 S上也形成了斑块。 typhosa 杂种宿主，具有一系列包含噬菌体21 N基因的λ i21 杂种噬菌体。这些结果对以沙门氏菌物种为宿主的意义讨论λ。

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