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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Comparative Evaluation of AntimicrobialEfficacy of Chlorhexidine, MTAD andChitosan as Root Canal Irrigantagainst Enterococcus faecalis
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Comparative Evaluation of AntimicrobialEfficacy of Chlorhexidine, MTAD andChitosan as Root Canal Irrigantagainst Enterococcus faecalis

机译：氯己定，MTAD和壳聚糖作为根肠灌肠液对粪肠球菌的抗菌作用的比较评价

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Endodontic irrigants play a role in disinfection of root canal. Inspite of wider options on selection of irrigant, there is always a search on ideal antibacterial irrigant against recurrent infections.Aim: To comparatively evaluate the antibacterial efficacy of chlorhexidine, Mixture of Tetracycline, Acid and Detergent (MTAD) and Chitosan against Enterococcus faecalis when used as a root canal irrigant.Materials and Methods: The bacterial E. faecalis culture was grown overnight in Brain Heart Infusion (BHI) broth and inoculated in Mueller-Hinton agar plates. The root canal irrigants were divided into four groups as follows. (Group I)-4% Sodium hypochlorite solutions; (Group II)-2% Chlorhexidine (CHX); (Group III)-MTAD; (Group IV)-2% Chitosan. Bacterial inhibition was assessed using agar well diffusion method. All four study irrigants were added to respective wells in agar plate (n=10) and incubated at 37°C for 24 hour. Diameter of bacterial inhibition zone around each well was recorded. The results obtained were statistically evaluated using one-way ANOVA test and the intergroup comparison was done using student?s t-test.Results: All the materials had statistically significant difference in zone of bacterial inhibition when compared to other materials. Based on the mean diameters, 4% sodium hypochlorite had the least zone of inhibition and MTAD had the highest zone of inhibition. 2% Chitosan polymer had greater zone of inhibition than 4% sodium hypochlorite, but less than 2% CHX and MTAD.Conclusion: MTAD showed the highest antibacterial efficacy against Enterococcus faecalis followed by CHX and Chitosan.
机译：牙髓冲洗剂在根管消毒中发挥作用。尽管在选择冲洗剂方面有更多选择，但始终都在寻找针对反复感染的理想抗菌冲洗剂。目标：为了比较评估洗必泰，四环素，酸和洗涤剂混合物（MTAD）和壳聚糖对肠球菌的抗菌效果粪便用作根管冲洗剂。材料和方法：细菌粪便大肠杆菌培养物在脑心浸液（BHI）肉汤中生长过夜，并接种在Mueller-Hinton琼脂平板中。根管冲洗液分为以下四组。 （I组）-4％次氯酸钠溶液; （第二组）-2％氯己定（CHX）; （第三组）-MTAD; （第四组）-2％壳聚糖。使用琼脂井扩散法评估细菌抑制作用。将所有四种研究冲洗液添加到琼脂平板（n = 10）的各个孔中，并在37°C下孵育24小时。记录每个孔周围的细菌抑制区的直径。使用单向方差分析对获得的结果进行统计学评估，并使用学生t检验进行组间比较。结果：与其他材料相比，所有材料在细菌抑制区域上均具有统计学上的显着差异。基于平均直径，4％的次氯酸钠具有最小的抑制范围，而MTAD具有最大的抑制范围。 2％的壳聚糖聚合物比4％的次氯酸钠具有更大的抑菌圈，但少于2％的CHX和MTAD。结论：MTAD对粪肠球菌的抗菌作用最高，其次是CHX和壳聚糖。

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