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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure
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Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure

机译：根据选择方法精子DNA片段化的频率：微流控设备和游泳程序的比较和相关性

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Introduction: Multiple rounds of centrifugation or washing spermatozoa can cause sperm DNA fragmentation (SDF); however, a microfluidic approach to select spermatozoa does not require centrifugation. Reports have suggested that sperm sorting using a microfluidic device is an effective method to select good quality spermatozoa, however, it is not known whether it reduces sperm DNA damage. We investigated whether the frequency of SDF was affected by selection method during sperm processing. Materials and Methods: Semen samples from ten men with normal, oligozoospermia and asthenozoospermia were split into two groups and sorted using a microfluidic device or by a swim-up method. Subsequently, semen parameters and SDF were measured and analyzed using paired or non-paired Student?s t-tests. Results: For samples sorted by the microfluidic device (Sperm Sorter Qualis?; Menicon, Kasugai, Japan) or the swim-up method, both showed a decrease in SDF. However, the decrease was more significant when the microfluidic device was used. Conclusion: Sorting using the microfluidic device resulted in less SDF than did the swim-up method.
机译：简介：多轮离心或洗涤精子会导致精子DNA片段化（SDF）;然而，选择精子的微流体方法不需要离心。有报告表明，使用微流控装置进行精子分选是选择优质精子的有效方法，但是，尚不清楚它是否能减少精子DNA损伤。我们调查了SDF的频率是否受精子处理过程中选择方法的影响。材料和方法：将来自正常，少精症和弱精子症的十名男性的精液样本分成两组，并使用微流控仪或游泳法进行分类。随后，使用配对或非配对的Student t检验测量并分析精液参数和SDF。结果：对于通过微流体装置（Sperm Sorter Qualis？; Menicon，Kasugai，Japan）或游泳法进行分选的样品，SDF均降低。然而，当使用微流体装置时，下降更为显着。结论：使用微流控装置进行分选的SDF少于游泳法。

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