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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Plasma Neutrophil Elastase and its Endogenous Inhibitors as Differential Inflammatory Markers for Dengue and Pneumonia
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Plasma Neutrophil Elastase and its Endogenous Inhibitors as Differential Inflammatory Markers for Dengue and Pneumonia

机译：血浆中性粒细胞弹性蛋白酶及其内源性抑制剂作为登革热和肺炎的不同炎症标记

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Neutrophils play a crucial role in protecting the host against microbial pathogens but they produce proteolytic enzymes such as Neutrophil Elastase (NE), the uncontrolled activity of which can destroy tissue and lead to organ failure. Thus, measurement of circulating levels of NE and its endogenous inhibitors may possibly contribute to diagnosis and management of diseases.Aim: This study was aimed to evaluate the changes in levels of NE, alpha1-Antitrypsin (a1-AT), alpha2-Macroglobulin (a2-MG) as well as elastase in complex with a1-AT (NE-a1-AT complex) in patients with dengue and pneumonia to ascertain if they could be of use as differential inflammatory markers and as adjunct diagnostic parameters in these two distinct disease conditions.Materials and Methods: This was a comparison study including 152 individuals in which 50 were in dengue group, 50 in pneumonia group, and 52 in control group. NE was measured using N-Succinyl-tri-alanine-p-nitroanilide as substrate. a1-AT, a2-MG and NE-a1-AT complex were estimated by Enzyme-Linked Immunosorbent Assay (ELISA). ANOVA, Kruskal-Wallis test and Pearson?s correlation tests were used to analyse the data. The results were expressed as Mean±SD and p-value <0.001 was considered statistically highly significant.Results: The result analysis indicated that the dengue and pneumonia patients had significantly higher elastase activity with significantly reduced a1-AT levels compared to controls. a2-MG level were significantly decreased in dengue while the levels were significantly increased in pneumonia.Conclusion: Significantly elevated levels of NE were observed in patients with dengue and pneumonia. Significantly reduced a1-AT in dengue and significantly increased a2-MG in pneumonia are observations of relevance.
机译：中性粒细胞在保护宿主抵抗微生物病原体方面起着至关重要的作用，但它们会产生蛋白水解酶，例如中性粒细胞弹性蛋白酶（NE），其活动不受控制会破坏组织并导致器官衰竭。因此，测量NE及其内源性抑制剂的循环水平可能有助于疾病的诊断和处理。目的：本研究旨在评估NE，α1-抗胰蛋白酶（a1-AT），α2的水平变化-登革热和肺炎患者中的巨球蛋白（a2-MG）以及弹性蛋白酶与a1-AT的复合物（NE-a1-AT复合物），以确定它们是否可用作鉴别性炎症标志物和这些疾病的辅助诊断参数两种不同的疾病状况。材料与方法：这项比较研究包括152个个体，其中登革热组50例，肺炎组50例，对照组52例。使用N-琥珀酰基-三丙氨酸-对硝基苯胺作为底物测量NE。通过酶联免疫吸附测定（ELISA）估算a1-AT，a2-MG和NE-a1-AT复合物。方差分析，Kruskal-Wallis检验和Pearson相关检验用于分析数据。结果表示为平均值±标准差，p值<0.001被认为是统计学上高度显着的。结果：结果分析表明，与对照组相比，登革热和肺炎患者的弹性蛋白酶活性明显更高，而a1-AT水平明显降低。登革热中a2-MG水平显着降低，而肺炎中a2-MG水平显着升高。结论：登革热和肺炎患者的NE水平显着升高。相关性观察到登革热中a1-AT显着降低，肺炎中a2-MG显着增加。

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