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首页> 外文期刊>Journal of bacteriology >GENETIC TRANSDUCTION WITH STAPHYLOPHAGE
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GENETIC TRANSDUCTION WITH STAPHYLOPHAGE

机译:葡萄球菌的遗传转化

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摘要

Korman, Ruth Z. (Elmira College, Elmira, N.Y.) and David T. Berman. Genetic transduction with staphylophage. J. Bacteriol. >84:228–236. 1962.—Transduction by crude lysates obtained by ultraviolet-light induction of phage 53 lysogenic derivatives of Staphylococcus aureus NCTC 8511 has been confirmed. A defined medium and sugar-differential medium were developed, permitting demonstration of transmission of sugar-fermentation loci. The range of transmissible markers has been extended to include free-coagulase production as well as antibiotic-resistance markers. A rare class of heterogeneous streptomycin-resistant transformed clones was obtained. These were assumed to be heterogenotic.
机译:Korman,Ruth Z.(纽约州Elmira的Elmira学院)和David T.Berman。带有噬菌体的基因转导。 J.细菌。 > 84: 228–236。 1962年。已确认通过紫外光诱导的金黄色葡萄球菌NCTC 8511噬菌体53溶原性衍生物对粗裂解物的转导。开发了确定的培养基和糖差异培养基,可以证明糖发酵基因座的传播。可传播标志物的范围已扩大到包括自由凝结酶生产以及抗生素抗性标志物。获得了罕见的一类异源链霉素抗性转化克隆。这些被认为是异质的。

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