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首页> 外文期刊>Journal of bacteriology >Cell-free Amino Acid-incorporating Systems from Bacillus licheniformis and Bacillus stearothermophilus 10
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Cell-free Amino Acid-incorporating Systems from Bacillus licheniformis and Bacillus stearothermophilus 10

机译：地衣芽孢杆菌和嗜热脂肪芽孢杆菌的无细胞氨基酸掺入系统10

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Cell-free amino acid-incorporating systems were prepared from log-phase cells of Bacillus licheniformis and B. stearothermophilus 10. No differences were observed between the two systems with respect to the types and relative amounts of the ribosomes, the time dependence of incorporation, and the optimal magnesium ion concentration (4 mm). The B. licheniformis system showed greater dependence on added transfer ribonucleic acid (tRNA) and was less stimulated by polyuridylic acid (poly U) than the B. stearothermophilus 10 system. Neither system was saturated at a level of 450 μg of poly U per incubation mixture. The S-100 fraction and the isolated tRNA inhibited incorporation in the B. stearothermophilus 10 system at 55 C but not at 37 C. No such inhibition was observed in the B. licheniformis system at either temperature. The tRNA prepared from B. subtilis, B. licheniformis, or B. stearothermophilus 10 could be used interchangeably in the two cell-free systems.
机译：用地衣芽孢杆菌和芽孢杆菌对数期细胞制备无细胞氨基酸掺入系统。 10.两种系统在核糖体的类型和相对量，掺入的时间依赖性以及最佳镁离子浓度（4 mm）方面均未观察到差异。 B。地衣形系统对添加的转移核糖核酸（tRNA）的依赖性更大，与 B相比，聚尿酸（poly U）的刺激较少。嗜热链球菌 10系统。每种培养混合物中的两个系统都未达到450 Ug聚U的饱和水平。 S-100级分和分离的tRNA抑制了掺入 B。硬脂嗜热菌 10系统在55°C而不是37°C下出现。在 B中未观察到这种抑制作用。两种温度下的地衣形系统。从 B制备的tRNA。枯草杆菌，地衣芽孢杆菌或 B。嗜热脂肪热菌 10可在两个无细胞系统中互换使用。

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