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首页> 外文期刊>Journal of bacteriology >Effect of Potassium Chloride on the Uptake and Storage of Phosphate by Saccharomyces mellis
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Effect of Potassium Chloride on the Uptake and Storage of Phosphate by Saccharomyces mellis

机译：氯化钾对啤酒酵母吸收和贮藏磷酸盐的影响

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The inorganic and polyphosphate pools of Saccharomyces mellis, grown in a medium containing excess phosphate, remain associated with the cells when the cells are suspended in a saline medium. If the cells are incubated in a medium containing 2 m KCl, the cells are altered in some manner which permits most of the orthophosphate and approximately one-third of the polyphosphate to be subsequently eluted by osmotic shock. At lower salt concentrations, β-mercaptoethanol enhances this salt effect but is inactive by itself in this respect. At equivalent ionic strengths, the sodium salt of ethylenediaminetetraacetic acid behaves exactly like KCl or any other monovalent ionic compound in altering the cell to susceptibility to osmotic shock. No special effect of this anion at either high or low concentration could be detected. Resting cells are refractory to being altered in this manner by salts if an energy source, such as glucose, is included in the reaction mixture. Cells which are depleted of phosphate reserves will immediately incorporate phosphate when suspended in a medium containing inorganic phosphate and an energy source. These cells exhibit the phenomenon of “überkompensation.” In resting cells, the inclusion of KCl in the reaction mixture prevents the conversion of orthophosphate into polyphosphate and, also, gradually decreases the ability of the organism even to assimilate orthophosphate. This effect is reversible, however, since the cells will incorporate phosphate in a normal manner if the cells are transferred to a non-salinized medium, or if a nitrogen source is included in the salinized reaction mixture so that the cells are now in a medium adequate for growth.
机译：在含过量磷酸盐的培养基中生长的的无机和多磷酸盐池，当细胞悬浮于盐溶液中时仍与细胞结合。如果将细胞在含有2 m KCl的培养基中孵育，则会以某种方式改变细胞，从而允许大部分正磷酸盐和大约三分之一的多磷酸盐随后通过渗透压洗脱。在较低的盐浓度下，β-巯基乙醇会增强这种盐效果，但在这方面本身是无活性的。在相等的离子强度下，乙二胺四乙酸钠盐的行为与KCl或任何其他一价离子化合物完全相同，可改变细胞对渗透性休克的敏感性。在高浓度或低浓度下均未检测到该阴离子的特殊作用。如果反应混合物中包含能量源，例如葡萄糖，则静止细胞难于被盐以这种方式改变。耗尽磷酸盐储备的细胞将悬浮在含有无机磷酸盐和能源的培养基中后立即掺入磷酸盐。这些细胞表现出“überkompensation”现象。在静止的细胞中，反应混合物中包含KCl会阻止正磷酸盐转化为多磷酸盐，并且甚至逐渐降低了生物体甚至吸收正磷酸盐的能力。但是，这种效果是可逆的，因为如果将细胞转移到非盐化培养基中，或者如果盐化反应混合物中包含氮源，则细胞会以正常方式掺入磷酸盐，从而使细胞现在处于培养基中足够增长。

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