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首页> 外文期刊>Journal of bacteriology >Peptidoglycan synthesis and turnover in cell division mutants of Agmenellum.
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Peptidoglycan synthesis and turnover in cell division mutants of Agmenellum.

机译：Agmenellum细胞分裂突变体中的肽聚糖合成和更新。

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摘要

The synthesis and turnover of peptidoglycan in Agmenellum quadruplicatum was investigated using D-[U-14C]alanine followed by proteolytic digestion. The rate of turnover of alanine in the peptide portion of the peptidoglycan was measured in strain BG-1 and in two division mutants of this strain: one was blocked in cell separation; and the other was a low-temperature, conditional cell division mutant. The peptide portion of peptidoglycan turned over in all three strains tested, but no correlation was observed between septum formation or cell separation and the rate of turnover. Peptidoglycan synthesis was measured during induced division in snake forms of strain SN-29. A stimulation of peptidoglycan synthesis was observed during the period of cross-wall formation, even in the absence of new protein synthesis. Thus in A. quadruplicatum, cross-wall synthesis is accompanied by a stimulation of peptidoglycan synthesis.
机译：使用D- [U-14C]丙氨酸，然后进行蛋白水解消化，研究了四芒草中肽聚糖的合成和周转。在菌株BG-1和该菌株的两个分裂突变体中，测定了肽聚糖的肽部分中丙氨酸的周转率。另一个是低温条件性细胞分裂突变体。肽聚糖的肽部分在所有测试的三个菌株中均发生翻转，但在隔膜形成或细胞分离与周转率之间未观察到相关性。在诱导分裂为SN-29毒蛇形式的过程中，测定了肽聚糖的合成。即使在没有新的蛋白质合成的情况下，在跨壁形成期间也观察到肽聚糖合成的刺激。因此，在四倍体农杆菌中，跨壁合成伴随着肽聚糖合成的刺激。

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