Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid.

N Keisel; B Vold

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Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid.

机译：来自枯草芽孢杆菌松弛菌株的甲基化不足的转移核糖核酸：菌株的构建和转移核糖核酸的分析。

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A strain of Bacillus subtilis is described from which undermethylated transfer ribonucleic acid (tRNA) can be obtained. The tRNA's from a methionine-limited culture were compared with those from a control culture with respect to general nucleoside composition, methylated components, and amino acid acceptor activity. The undermethylated tRNA's had the normal amounts of the four major nucleosides, pseudouridine, and 5-methyluridine (ribothymidine), but were deficient in methylated nucleosides other than 5-methyluridine. These methyl-deficient nucleosides can be fully remethylated in the presence of the appropriate methylases. Since the majority of the work characterizing undermethylated tRNA's has been done using Escherichia coli, the work with B. subtilis presents some interesting comparisons and offers an alternative substrate for methylase studies.

机译：描述了一种枯草芽孢杆菌菌株，可从其中获得甲基化不足的转移核糖核酸（tRNA）。将蛋氨酸限制培养物中的tRNA与对照培养物中的tRNA进行比较，以了解其总体核苷组成，甲基化成分和氨基酸受体活性。甲基化不足的tRNA具有正常含量的四个主要核苷，假尿苷和5-甲基尿苷（核苷胸苷），但除5-甲基尿苷外甲基化的核苷不足。这些甲基不足的核苷可以在适当的甲基化酶存在下完全重甲基化。由于大多数表征欠甲基化tRNA的工作都是使用大肠杆菌完成的，因此与枯草芽孢杆菌的工作提出了一些有趣的比较，并为甲基化酶研究提供了另一种底物。

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《Journal of bacteriology》 |1976年第1期|共6页
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N Keisel; B Vold;
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中图分类微生物学;
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1. Analysis of Isoaccepting Transfer Ribonucleic Acid Species of Bacillus subtilis: Changes in Chromatography of Transfer Ribonucleic Acids Associated with Stage of Development [J] . Barbara S. Vold Journal of bacteriology . 1973,第1期

机译：枯草芽孢杆菌同等接受转移核糖核酸物种的分析：与发展阶段相关的转移核糖核酸的色谱变化
2. In Vivo Aminoacylation of Transfer Ribonucleic Acid in Bacillus subtilis and Evidence for Differential Utilization of Lysine-Isoaccepting Transfer Ribonucleic Acid Species [J] . Judith Tockman, Barbara S. Vold Journal of bacteriology . 1977,第3期

机译：枯草芽孢杆菌中转移核糖核酸的体内氨基酰化作用和赖氨酸同工接受转移核糖核酸物种的差异利用的证据
3. Alteration of tyrosine isoaccepting transfer ribonucleic acid species in wild-type and asporogenous strains of Bacillus subtilis. [J] . R A McMillian, J L Arceneaux Journal of bacteriology . 1975,第2期

机译：枯草芽孢杆菌的野生型和产孢菌株中酪氨酸同接受转移核糖核酸种类的变化。
4. Studies on the Mechanism of the Aminoacylation Reaction of Transfer Ribonucleic Acid at High Pressure [C] . J. Barciszewski, P. Krzeminski, A. Wandel, International conference on high pressure bioscience and biotechnology . 1999

机译：转移核糖核酸在高压下的氨基化反应机理研究
5. SPECTROSCOPIC AND PHYSICAL STUDIES OF THE STRUCTURE OF BACILLUS SUBTILIS RIBOSOMAL 5S RIBONUCLEIC ACID (NMR, NUCLEAR OVERHAUSER EFFECT, INFRARED, CALORIMETRY). [D] . CHANG, LEE-HONG. 1985

机译：芽孢杆菌核糖体5S核糖核酸的结构的光谱学和物理研究（NMR，核超载效应，红外，量热法）。
6. Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid. [O] . N Keisel, B Vold 1976

机译：来自枯草芽孢杆菌松弛菌株的甲基化转移核糖核酸：菌株的构建和转移核糖核酸的分析。
7. Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid [O] . N Keisel, B Vold 1976

机译：来自枯草芽孢杆菌的松弛菌株的脱甲酰化的转移核糖核酸：构建菌株和转移核糖核酸的分析
8. Circular Dichroism Study of Nine Species of Transfer Ribonucleic Acid. [R] . blum, arlene diane 1972

机译：9种转移核糖核酸的圆二色谱研究。

Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid.

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