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首页> 外文期刊>Journal of bacteriology >Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.
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Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.

机译:缺乏硫氧还蛋白还原酶的大肠杆菌突变体的分离。

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摘要

A mutant of Escherichia coli defective in thioredoxin reductase has been isolated and partially characterized. This mutant has no detectable thioredoxin reductase activity in vitro and yet it exhibits no in vivo defect in reduction of ribonucleotides. Evidence is presented that indicates that, in cells permeabilized via ether treatment, ribonucleoside diphosphate reduction can utilize glutathione as an alternate reducing system.
机译:硫氧还蛋白还原酶缺陷的大肠杆菌突变体已被分离并部分鉴定。该突变体在体外没有可检测的硫氧还蛋白还原酶活性,但在核糖核苷酸的还原中没有表现出体内缺陷。提出的证据表明,在通过醚处理透化的细胞中,核糖核苷二磷酸还原可以利用谷胱甘肽作为替代的还原系统。

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