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首页> 外文期刊>Journal of bacteriology >Production of large amounts of acetate during germination of Bacillus megaterium spores in the absence of exogenous carbon sources.
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Production of large amounts of acetate during germination of Bacillus megaterium spores in the absence of exogenous carbon sources.

机译：在没有外源碳源的情况下，巨大芽孢杆菌孢子萌发过程中产生大量乙酸盐。

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摘要

When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into the medium. Exogenous pyruvate and alanine are also converted to CO2 and acetate by germinating spores, presumably by using the pyruvate dehydrogenase that is present in dormant spores. These data suggest that the 3-phosphoglyceric acid stores in the dormant spore and alanine generated by proteolysis early in germination can be catabolized to acetate during germination with production of large amounts of reduced nicotinamide adenine dinucleotide, acetyl coenzyme A, and adenosine 5'-triphosphate.
机译：当巨大芽孢杆菌的孢子在没有外源碳源的情况下发芽时，发芽的最初几分钟伴随着大量乙酸的产生（大约70 nmol / mg干孢子）乙酸盐以及少量的丙酮酸和乳酸。这些化合物大部分都被排泄到培养基中。外源的丙酮酸和丙氨酸也可以通过萌发芽孢而转化为二氧化碳和乙酸盐，大概是利用休眠孢子中存在的丙酮酸脱氢酶。这些数据表明在萌发过程中萌发初期通过蛋白水解产生的休眠孢子和丙氨酸中的3-磷酸甘油存储可以在萌发过程中分解为乙酸盐，并产生大量还原的烟酰胺腺嘌呤二核苷酸，乙酰辅酶A和5'-三磷酸腺苷。 。

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