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首页> 外文期刊>Journal of bacteriology >Effect of cyclic guanosine 3',5'-monophosphate on nitrogen fixation in Rhizobium japonicum.
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Effect of cyclic guanosine 3',5'-monophosphate on nitrogen fixation in Rhizobium japonicum.

机译：环状鸟苷3'，5'-单磷酸对日本根瘤菌固氮的影响。

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摘要

The addition of exogenous cyclic guanosine 3',5'-monophosphate (cGMP) at a concentration of 0.1 mM to a free-living culture of Rhizobium japonicum 3I1b110 was found to completely inhibit the expression of nitrogenase activity and markedly inhibit the expression of hydrogenase and nitrate reductase activities. The effect was specific for cGMP. Experiments on the in vivo incorporation of radioactive methionine and subsequent analysis of the labeled proteins on polyacrylamide gels showed that the biosynthesis of nitrogenase polypeptides was inhibited. It appears that the time of addition of cGMP is important since the effect was only seen during the early stages of nif gene expression. The intracellular level of cGMP was found to respond to physiological changes in the cell, and there was a fall in cGMP concentrations when nitrogenase was induced. Microaerophilic-aerobic shift experiments showed that intracellular levels increased from 0.25 pmol/mg of cell protein under microaerophilic conditions to 2.6 pmol/mg of cell protein under aerobic conditions, suggesting that the cellular pool size of cGMP may be under redox control.
机译：发现将0.1 mM浓度的外源环状鸟苷3'，5'-单磷酸（cGMP）添加到日本根瘤菌3I1b110的自由生活培养物中可以完全抑制固氮酶活性的表达，并显着抑制氢化酶和硝酸还原酶活性。该作用对cGMP是特异的。在体内掺入放射性蛋氨酸的实验以及随后在聚丙烯酰胺凝胶上分析标记的蛋白质的实验表明，固氮酶多肽的生物合成受到抑制。似乎添加cGMP的时间很重要，因为这种作用仅在nif基因表达的早期才可见。发现细胞内cGMP水平对细胞中的生理变化有反应，当诱导固氮酶时，cGMP浓度会下降。微需氧-好氧转化实验表明，细胞内水平从微需氧条件下的0.25 pmol / mg细胞蛋白增加到好氧条件下的2.6 pmol / mg细胞蛋白，表明cGMP的细胞池大小可能受氧化还原控制。

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