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首页> 外文期刊>Journal of bacteriology >Segregation of Bacillus subtilis chromosomes radioactively labeled during the first round of replication after germination of spores.
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Segregation of Bacillus subtilis chromosomes radioactively labeled during the first round of replication after germination of spores.

机译：枯草芽孢杆菌染色体的分离在孢子萌发后的第一轮复制过程中被放射性标记。

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Spores of Bacillus subtilis W23 thy his were allowed to incorporate [3H]thymine for short periods of time either continuously from, or soon after, the start of the first round of replication after germination. They were then transferred to nonradioactive medium to allow growth into microcolonies (up to 12 cells), which were autoradiographed. The relative numbers of various types (major versus minor) of grain clusters associated with individual microcolonies throughout the populations were scored. Analysis of the results showed clearly that, in the majority of spores at least, only one chromosome was undergoing replication soon after the start of deoxyribonucleic acid synthesis. Furthermore, under the conditions used, no evidence for initiation of replication of a second chromosome within 25 min after the first could be obtained. Accepting that B. subtilis spores are essentially homogenous in deoxyribonucleic acid content, the results support the conclusion that the spore contains only one copy of the chromosome, not two.
机译：枯草芽孢杆菌W23的孢子可以在发芽后第一轮复制开始后或之后的短时间内掺入[3H]胸腺嘧啶。然后将它们转移到非放射性培养基中，使其生长成可进行放射自显影的微菌落（最多12个细胞）。对整个种群中与单个微殖民地相关的各种类型（主要和次要）的谷物簇的相对数量进行了评分。对结果的分析清楚地表明，至少在大多数孢子中，在脱氧核糖核酸合成开始后不久就有一个染色体正在复制。此外，在所使用的条件下，无法获得在第一条染色体后25分钟内第二条染色体开始复制的证据。接受枯草芽孢杆菌的孢子在脱氧核糖核酸含量上基本上是同质的，结果支持了这样的结论，即孢子仅包含一个染色体拷贝，而不包含两个拷贝。

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