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首页> 外文期刊>Journal of bacteriology >Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid.
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Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid.

机译:大肠杆菌K-12突变的分离和遗传特征,这些突变影响ColIb质粒对噬菌体T5的限制。

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A mutant derivative of Escherichia coli K-12 has been isolated which is permissive for bacteriophage T5 infection even when harboring a wild-type ColIb plasmid. The fully permissive phenotype was the result of two mutations that are located near the rpsL-rpsE region on the E. coli chromosome and are recessive to the wild-type alleles. These mutations had little or no effect on induction of colicin synthesis and did not affect the expression of antibiotic resistance by the resistance plasmids R64drd11 or R1drd19. Cells harboring the mutant alleles grew more slowly than isogenic wild-type derivatives in either minimal or complete media.
机译:已经分离出大肠杆菌K-12的突变体衍生物,即使携带野生型ColIb质粒,该突变体也允许噬菌体T5感染。完全允许的表型是两个突变的结果,这两个突变位于大肠杆菌染色体上的rpsL-rpsE区附近,对野生型等位基因是隐性的。这些突变对大肠菌素合成的诱导几乎没有或没有影响,并且不影响抗性质粒R64drd11或R1drd19的抗生素抗性表达。在基本或完全培养基中,具有突变等位基因的细胞比等基因野生型衍生物生长得慢。

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