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首页> 外文期刊>Journal of bacteriology >Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium.
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Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium.

机译：大肠杆菌和鼠伤寒沙门氏菌趋化性基因的功能同源性。

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Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime elements. Wild-type che genes of E. coli F'420 complemented all che mutants of Salmonella except cheS, cheV, and a subclass of cheU. A series of tester episomes carrying E. coli che mutations were then used to determine which E. coli che function was responsible for the complementation of each Salmonella che defect. By this method, the following correspondences were determined: cheAE and chePS,cheWE, and cheWS, cheXE and cheRS, cheYE and cheQS, cheCE and cheUS, cheBE and cheXS, and cheZE and cheTS. (The subscripts E and S refer to E. coli and S. typhimurium genes, respectively.) In some tests, especially those involving the last two pairs of genes, poor complementation was observed between noncorresponding genes. A model explaining these observations in terms of subunit interactions is proposed.
机译：通常，通过种间互补试验分析大肠杆菌和鼠伤寒沙门氏菌的非趋化突变体，以确定这两种生物的che基因之间的功能对应。通过一系列F-prime元件将大肠杆菌che区域引入沙门氏菌受体。大肠杆菌F'420的野生型che基因补充了除cheS，cheV和cheU的一个亚类以外的沙门氏菌的所有che突变体。然后使用一系列携带大肠杆菌che突变的测试附加体来确定哪种大肠杆菌che功能负责每个沙门氏菌che缺陷的互补。通过这种方法，确定了以下对应关系：cheAE和chePS，cheWE和cheWS，cheXE和cheRS，cheYE和cheQS，cheCE和cheUS，cheBE和cheXS以及cheZE和cheTS。 （下标E和S分别指大肠杆菌和鼠伤寒沙门氏菌基因。）在某些测试中，尤其是涉及最后两对基因的测试，在不对应的基因之间观察到互补性差。提出了用亚基相互作用解释这些观察结果的模型。

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