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首页> 外文期刊>Journal of bacteriology >Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside.
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Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside.

机译:在高浓度核苷存在下生长的大肠杆菌K-12菌株RR1和chi1776中pBR322质粒脱氧核糖核酸的扩增增加。

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摘要

When pBR322 plasmid-harboring Escherichia coli strains RR1 or chi1776 were grown in the presence of 1 mg of uridine or cytidine per ml and later treated with chloramphenicol, as much as three times more plasmid deoxyribonucleic acid was recovered than would normally be obtained by routine plasmid amplification procedures.
机译:当携带pBR322质粒的大肠杆菌RR1或chi1776菌株在每毫升1 mg尿苷或胞苷存在下生长并随后用氯霉素处理时,回收的质粒脱氧核糖核酸的回收量是常规质粒正常回收的三倍扩增程序。

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