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首页> 外文期刊>Journal of bacteriology >Acceptance by Erwinia spp. of R Plasmid R68.45 and Its Ability to Mobilize the Chromosome of Erwinia chrysanthemi
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Acceptance by Erwinia spp. of R Plasmid R68.45 and Its Ability to Mobilize the Chromosome of Erwinia chrysanthemi

机译：Erwinia spp接受。质粒R68.45的克隆及其动员菊花欧文氏体染色体的能力

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R plasmid R68.45 was transferred in broth matings from Escherichia coli to strains of Erwinia amylovora, E. carotovora subsp. atroseptica, E. chrysanthemi, and E. herbicola (Enterobacter agglomerans); the frequency of transfer ranged from 2 × 10?8 to 5 × 10?4 per input donor cell depending on the bacterial species. The drug resistance markers tet+, amp+, and kan+ were stable in these Erwinia species. Transconjugants of Erwinia spp., but not of the wild-type parent Erwinia strains, acquired levels of antibiotic resistance (tetracycline, 50 μg/ml; ampicillin, 200 μg/ml; kanamycin 200 μg/ml) similar to those of the donor R68.45-bearing strain of Escherichia coli. Erwinia transconjugants (with one exception of E. carotovora subsp. atroseptica) were donors of the antibiotic resistance markers; the frequency of transfer was consistently higher with an E. coli strain than with Erwinia spp. as recipients, and when matings were done on a solid surface (membranes) rather than in liquid. Transfer of chromosomal markers ade+, gal+, gtu+ (utilization of galacturonate), his+, leu+, lys+, thr+, and trp+ occurred in crosses between E. chrysanthemi strains harboring R68.45 and appropriate recipient strains; the frequency of transfer ranged from 9.0 × 10?8 to 2.0 × 10?6 depending on the selective marker. Analysis of the coinheritance of unselected markers among various classes of recombinants revealed linkage between thr-leu-lys-ade and between trp and his, thus confirming earlier findings with the Hfr-type donor cells. Since R68.45 mobilized an array of chromosomal markers in the wild-type as well as genetically marked strains of E. chrysanthemi, the system, used in conjunction with the existing Hfr strains, should provide a useful tool to study the genetics of plant pathogenicity of this bacterial species. In contrast to E. chrysanthemi, R68.45 did not mobilize chromosomal markers ilv+, his+, rbs+, ser+, and thr+ in E. amylovora EA178.
机译：将R质粒R68.45在大肠杆菌的肉汤交配中转移到淀粉小球藻（Erwinia amylovora），大肠杆菌（E. carotovora）亚种的菌株中。 atroseptica，E。chrysanthemi 和 E。 Herbicola （结团肠杆菌）;每个输入供体细胞的转移频率范围从2×10 ？8 到5×10 ？4 取决于细菌的种类。耐药标记 tet + ， amp + 和 kan + 在这些 Erwinia 物种中稳定。 Erwinia 菌种的转导结合体，而不是野生型亲本 Erwinia 菌株的转导结合体，获得的抗生素抗性水平（四环素为50μg/ ml;氨苄青霉素为200μg/ ml ;卡那霉素200μg/ ml）类似于带有大肠杆菌R68.45的供体菌株。欧文氏菌（Erwinia）转导结合剂（除 carotovora 亚种 toroseptica 外）是抗生素抗性标记物的供体。 E的转移频率始终较高。大肠杆菌菌株比 Erwinia spp。作为接受者，并且在固体表面（膜）而不是液体上进行交配。染色体标记 ade + ， gal + ， gtu +的转移（半乳糖醛酸酯的利用）， his + ， leu + ， lys + ， thr + 和 trp + 发生在< em> E。含有R68.45的Chrysanthemi 菌株和合适的受体菌株;根据选择标记的不同，转移的频率范围为9.0×10 ？8 到2.0×10 ？6 。对各种重组子之间未选择的标记的一致性进行分析，揭示了 thr-leu-lys-ade 之间以及 trp 和 his 之间的联系，因此证实了Hfr型供体细胞的早期发现。自R68.45以来，动员了野生型以及遗传标记的Eem菌株的一系列染色体标记。 chrysanthemi 系统与现有的Hfr菌株结合使用，应该为研究该细菌种的植物致病性遗传学提供一个有用的工具。与 E相反。 chrysanthemi ，R68.45没有动员染色体标记 ilv + ， his + ， rbs + ， ser + 和 thr + E。扁桃体EA178。

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