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首页> 外文期刊>Journal of bacteriology >Accumulation of Streptomycin-Phosphate in Cultures of Streptomycin Producers Grown on a High-Phosphate Medium
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Accumulation of Streptomycin-Phosphate in Cultures of Streptomycin Producers Grown on a High-Phosphate Medium

机译：在高磷酸盐培养基上生长的链霉素生产者培养物中磷酸链霉素的积累

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摘要

A phosphorylated derivative of streptomycin accumulated in cultures of Streptomyces griseus ATCC 12475 and SC2376 grown on complex media containing an excess of inorganic phosphate (0.01 m). This compound did not accumulate significantly in the absence of added inorganic phosphate. The phosphorylated derivative did not inhibit growth of Bacillus subtilis or support growth of a streptomycin-dependent strain of Escherichia coli; however, incubation of the derivative with alkaline phosphatase gave a compound which was active with both systems. In the phosphorylated derivative, phosphate is esterified with an —OH group of the streptidine moiety of streptomycin. It is suggested that the phosphoryl group is introduced during biosynthesis of the streptidine moiety of streptomycin or by the action of streptomycin kinase on preformed streptomycin (or both), and subsequent dephosphorylation by streptomycin-phosphate phosphatase is inhibited by high concentrations of inorganic phosphate. A column chromatographic procedure for separation of streptidine-phosphate, streptomycin-phosphate, and streptomycin is described.
机译：链霉素的磷酸化衍生物积累在 Streptomyces griseus ATCC 12475和SC2376的培养物中，该培养基生长在含有过量无机磷酸盐（0.01 m）的复杂培养基上。在不添加无机磷酸盐的情况下，该化合物没有明显积累。磷酸化的衍生物不会抑制枯草芽孢杆菌的生长，也不会支持链霉素依赖性的大肠杆菌的生长;但是，将该衍生物与碱性磷酸酶一起孵育后得到的化合物在两个系统上均处于活动状态。在该磷酸化衍生物中，磷酸被链霉素的链霉素部分的-OH基团酯化。有人建议在链霉素的链环部分的生物合成过程中或通过链霉素激酶对预先形成的链霉素（或两者）的作用引入磷酸基，随后高浓度的无机磷酸盐抑制链霉素-磷酸磷酸酶的去磷酸化作用。描述了用于分离磷酸链霉素，磷酸链霉素和链霉素的柱色谱方法。

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