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首页> 外文期刊>Journal of bacteriology >Multiple Forms of Pseudomonas multivorans Glucose-6-Phosphate and 6-Phosphogluconate Dehydrogenases: Differences in Size, Pyridine Nucleotide Specificity, and Susceptibility to Inhibition by Adenosine 5′-Triphosphate
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Multiple Forms of Pseudomonas multivorans Glucose-6-Phosphate and 6-Phosphogluconate Dehydrogenases: Differences in Size, Pyridine Nucleotide Specificity, and Susceptibility to Inhibition by Adenosine 5′-Triphosphate

机译：多种形式的假单胞菌葡萄糖6-磷酸和6-磷酸葡萄糖酸脱氢酶的形式：大小，吡啶核苷酸特异性和对5'-三磷酸腺苷抑制的敏感性的差异。

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Two major species of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) differing in size, pyridine nucleotide specificity, and susceptibility to inhibition by adenosine 5′-triphosphate (ATP) were detected in extracts of Pseudomonas multivorans (which has recently been shown to be synonymous with the species Pseudomonas cepacia) ATCC 17616. The large species (molecular weight ca. 230,000) was active with nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) and was markedly inhibited by ATP, which decreased its affinity for glucose-6-phosphate and for pyridine nucleotides. This form of the enzyme exhibited homotropic effects for glucose-6-phosphate. The small species (molecular weight ca. 96,000) was active with NADP but not with NAD, was not inhibited by ATP, and exhibited no homotropic effects for glucose-6-phosphate. Under certain conditions multiplicity of 6-phosphogluconate dehydrogenase (EC 1.1.1.43) activities was also noted. One form of the enzyme (80,000 molecular weight) was active with either NAD or NADP and was inhibited by ATP, which decreased its affinity for 6-phosphogluconate. The other form (120,000 molecular weight) was highly specific for NADP and was not susceptible to inhibition by ATP. Neither form of the enzyme exhibited homotropic effects for 6-phosphogluconate. The possible relationships between the different species of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase are discussed.
机译：在多假单胞菌提取物中提取到两种主要的葡萄糖-6-磷酸脱氢酶（EC 1.1.1.49），其大小，吡啶核苷酸特异性以及对腺苷5'-三磷酸（ATP）的抑制敏感性不同。 >（最近已证明与洋葱假单胞菌（Pseudomonas cepacia）同义）ATCC17616。大物种（分子量约23万）与烟酰胺腺嘌呤二核苷酸（NAD）或烟酰胺腺嘌呤二核苷酸磷酸有活性。 （NADP）并被ATP显着抑制，从而降低了其对6磷酸葡萄糖和吡啶核苷酸的亲和力。这种形式的酶对6-磷酸葡萄糖表现出同质作用。这种小分子（分子量约为96,000）对NADP具有活性，但对NAD不具有活性，不受ATP的抑制，并且对6-磷酸葡萄糖没有同质作用。在某些条件下，还注意到6-磷酸葡糖酸脱氢酶（EC 1.1.1.43）的多样性。一种形式的酶（分子量为80,000）对NAD或NADP均具有活性，并被ATP抑制，从而降低了其对6-磷酸葡萄糖酸酯的亲和力。另一种形式（分子量为120,000分子量）对NADP具有高度特异性，并且不易受ATP抑制。两种形式的酶都没有表现出对6-磷酸葡萄糖酸酯的同质作用。讨论了不同种类的葡萄糖6-磷酸脱氢酶和6-磷酸葡萄糖酸酯脱氢酶之间的可能关系。

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