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首页> 外文期刊>Journal of bacteriology >Fertility properties and regulation of antimicrobial substance production by plasmid SCP2 of Streptomyces coelicolor.
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Fertility properties and regulation of antimicrobial substance production by plasmid SCP2 of Streptomyces coelicolor.

机译：Coelicolor链霉菌SCP2的育性和对抗菌物质产生的调节。

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Streptomyces coelicolor A3(2) possesses two plasmids (SCP1 and SCP2) that act as sex factors. The plasmid deoxyribonucleic acid isolated from S. coelicolor A3(2) SCP1- strains A617 and A585 had the same molecular weight and endonuclease cleavage pattern as the SCP2 plasmid. The plasmidless strain S18 SCP2- was isolated from the A617 X A585 cross. SCP2 plasmid-containing strains acted as donors of chromosomal markers, whereas the plasmidless strain acted as recipient. The transfer of SCP2+ donor strain markers into the SCP2- recipient occurred at high frequencies (approximately 75%), was unidirectional, was initiated from a fixed region of the chromosome, and had the SCP2 fertility factor transferred first. The introduction of the SCP2 plasmid into a recipient strain greatly reduced the recombination frequency. These fertility properties differed from those previously reported, thereby suggesting that the SCP2 plasmid examined in this investigation may be an additional variant to those described in the literature. The SCP2 plasmid also regulated production of three antibacterial substances and conveyed resistance for S. coelicolor A3(2) strains against growth inhibition by one of them.
机译：天蓝色链霉菌A3（2）拥有两个作为性因子的质粒（SCP1和SCP2）。从大肠杆菌天蓝色链霉菌A3（2）SCP1-菌株A617和A585分离的质粒脱氧核糖核酸具有与SCP2质粒相同的分子量和核酸内切酶切割模式。从A617 X A585杂交分离出无质粒S18 SCP2-。含有SCP2质粒的菌株充当染色体标记的供体，而无质粒的菌株充当受体。 SCP2 +供体菌株标记物以高频率（约75％）转移到SCP2-受体中，是单向的，是从染色体的固定区域开始的，并且首先转移了SCP2的生育因子。将SCP2质粒引入受体菌株中大大降低了重组频率。这些育性特性不同于先前报道的那些，从而表明在这项研究中检查的SCP2质粒可能是文献中所述的另一种变异。 SCP2质粒还调节三种抗菌物质的产生，并传达了针对其中一种细菌对天蓝色链霉菌A3（2）菌株的生长抑制的抗性。

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