...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Isolation of nonsense suppressor mutants in Pseudomonas.
【24h】

Isolation of nonsense suppressor mutants in Pseudomonas.

机译：假单胞菌中无意义的抑制突变体的分离。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A strain of Escherichia coli harboring the drug resistance plasmid RP1 was treated with the mutagen N-methyl-N-nitro-N-nitro-N-nitrosoguanidine, and mutants were isolated in which ampicillin resistance had been lost due to an amber mutation in the plasmid. One of these mutants was again treated, and a strain was isolated in which tetracycline resistance was also lost due to an amber mutation in the plasmid. The plasmid containing amber mutations in the genes amp and tet was named pLM2. This plasmid could be transferred to strains of Pseudomonas aeruginosa, P. phaseolicola, and P. pseudoalcaligenes. Mutants resistant to ampicillin and tetracycline could not be obtained from P. phaseolicola carrying pLM2. However, strains of E. coli, P. aeruginosa, and P. pseudoalcaligenes carrying the plasmid did produce mutants simultaneously resistant to both antibiotics. All of the mutants of E. coli had developed nonsense suppressors since they became phenotypically lac+, although harboring a lac amber mutation, and formed plaques with amber mutants of phages PRR1 and PRD1 that attack organisms carrying RP1. Approximately 20% of the resistant mutants of P. aeruginosa and P. pseudoalcaligenes were sensitive to the amber mutant of PRD1. These mutants were of variable stability and grew somewhat more slowly than their parent strains. One of the suppressor mutants of P. pseudoalcaligenes, designated ERA(pLM2)S4, was used for the isolation of nonsense mutants of bacteriophage PHA6, a virus having a segmented genome of double-stranded ribonucleic acid and an envelope of lipids and proteins.
机译：用诱变剂N-甲基-N-硝基-N-硝基-N-亚硝基胍处理带有耐药质粒RP1的大肠杆菌菌株，分离出突变体，其中由于琥珀酸突变导致氨苄青霉素抗性丧失。质粒。再次处理这些突变体之一，并分离出其中由于质粒中的琥珀色突变而使四环素抗性丧失的菌株。在amp和tet基因中含有琥珀色突变的质粒称为pLM2。该质粒可以转移到铜绿假单胞菌，菜豆假单胞菌和假拟杯假单胞菌的菌株中。无法从携带pLM2的菜豆中获得抗氨苄青霉素和四环素的突变体。但是，携带该质粒的大肠杆菌，铜绿假单胞菌和假拟假单胞菌菌株确实产生了同时对两种抗生素具有抗性的突变体。大肠杆菌的所有突变体都表现出无意义的抑制子，因为它们虽然具有lac琥珀色突变，但在表型上却变成lac +，并与噬菌体PRR1和PRD1的琥珀色突变体形成噬斑，攻击了携带RP1的生物。大约20％的铜绿假单胞菌和假拟青霉的抗性突变体对PRD1的琥珀色突变体敏感。这些突变体具有可变的稳定性，并且比其亲本菌株生长得慢一些。一种伪拟疟原虫的抑制突变体，称为ERA（pLM2）S4，用于分离噬菌体PHA6的无意义突变体，该病毒具有分段的双链核糖核酸基因组和脂质和蛋白质包膜。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号