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首页> 外文期刊>Journal of bacteriology >Binding of plant lectins to mycoplasma cells and membranes.
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Binding of plant lectins to mycoplasma cells and membranes.

机译:植物凝集素与支原体细胞和膜的结合。

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The binding of iodinated wheat germ agglutinin, Ricinus communis agglutinin, and concanavalin A to mycoplasma cells and membranes was examined. All mycoplasmas studied specifically bound concanavalin A or R. communis agglutinin and, to a lesser degree, wheat germ agglutinin. The binding of lectins to whole cells was similar to that recorded for membranes, suggesting that significant binding only occurred on the outer surface of the mycoplasma membrane. Proteolysis of the membrane almost always increased the capacity to bind lectins, which indicates that additional carbohydrate groups on the mycoplasma membrane are masked by a protein layer or protein complexes on the membrane. The observation that carbohydrates are apparently exposed on the surface of mycoplasma membranes should stimulate more concentrated study on the isolation and chemical characterization of these substances since it is quite likely that they are responsible for a variety of reactions between mycoplasmas and host cells.
机译:检查了碘化小麦胚芽凝集素,Ricinus communis凝集素和伴刀豆球蛋白A与支原体细胞和膜的结合。所有研究的支原体均特异性结合伴刀豆球蛋白A或R. communis凝集素,并在较小程度上结合小麦胚芽凝集素。凝集素与全细胞的结合与膜所记录的相似,表明显着的结合仅发生在支原体膜的外表面。膜的蛋白水解几乎总是增加结合凝集素的能力,这表明支原体膜上的其他碳水化合物基团被膜上的蛋白质层或蛋白质复合物所掩盖。碳水化合物明显暴露于支原体膜表面的观察结果应促进对这些物质的分离和化学表征的更集中的研究,因为它们很可能负责支原体和宿主细胞之间的多种反应。

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