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首页> 外文期刊>Journal of bacteriology >Ribonucleic acid synthesis during fruiting body formation in Myxococcus xanthus.
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Ribonucleic acid synthesis during fruiting body formation in Myxococcus xanthus.

机译：黄果粘球菌子实体形成过程中的核糖核酸合成。

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A method has been devised that allowed us, for the first time, to pulse-label M. xanthus cells with precursors for ribonucleic acid biosynthesis while they were undergoing fruiting body formation. Using this method, we examined patterns of ribonucleic acid (RNA) accumulation throughout the process of fruiting body formation. As development proceeded, the rate of RNA accumulation increased at two periods of the developmental cycle: once just before aggregation and once late in the cycle, when sporulation was essentially completed. In contrast to vegetatively growing cells, in which only stable RNA species are labeled during a 30-min pulse, the majority of radioactivity found in RNA from 30-min pulse-labeled developing cells was found in an unstable heterodisperse fraction that migrated to the 5S to 16S region of sucrose density gradients and sodium dodecyl sulfate-polyacrylamide gels. This pattern of incorporation could not be induced (i) by a shift down of vegetatively growing cells to a nutritionally poor medium, in which the generation time was increased to that of developing cells during the growth phase, or (ii) by plating of vegetative cells onto the same solid-surface environment as that of developing cells, but which surface supported vegetative growth rather than fruiting body formation. Thus, the RNA synthesis pattern observed appeared to be related to development per se rather than to nutritional depletion or growth on a solid surface alone. The radioactivity incorporated into the unstable 5S to 16S RNA fraction accumulated as the pulse length was increased from 10 to 30 min; in contrast, an analogous unstable fraction from vegetative cells decreased as pulse length was increased. This suggested that developmental 5S to 16S RNA was more stable than vegetative cell 5S to 16S RNA (presumptive messenger RNA). However, during a 45-min chase period, radioactivity in 30-min-pulse-labeled developmental 5S to 16S RNA decayed to an extent twice that of developmental RNA located in 16S and 23S regions of sucrose density gradients and was considerably less stable than the 5S, 16S, and 23S RNA species labeled during a 30-min pulse of vegetative cells.
机译：已经设计出一种方法，这使我们第一次可以在核果核酸细胞进行子实体形成时用核糖核酸生物合成的前体对它们进行脉冲标记。使用这种方法，我们检查了子实体形成过程中核糖核酸（RNA）积累的模式。随着发育的进行，RNA积累的速率在发育周期的两个阶段增加：一次在聚集之前，一次在周期后期，当孢子形成基本完成时。与营养生长的细胞（在30分钟的脉冲中仅标记稳定的RNA物种）相反，在30分钟的脉冲标记的发育细胞中的RNA中发现的大部分放射性是在不稳定的杂散组分中迁移到5S的至蔗糖密度梯度的16S区和十二烷基硫酸钠-聚丙烯酰胺凝胶。 （i）营养生长的细胞向营养不良的培养基下移，不能诱导这种掺入模式，在营养不良的培养基中，生长时间增加到发育细胞的发育时间，或者（ii）营养平板接种将细胞置于与发育中的细胞相同的固体表面环境中，但是该表面支持营养生长而不是子实体的形成。因此，观察到的RNA合成模式似乎与发育本身有关，而不是与营养消耗或仅在固体表面上的生长有关。当脉冲长度从10分钟增加到30分钟时，并入到不稳定的5S至16S RNA馏分中的放射活性;相反，随着脉冲长度的增加，来自营养细胞的类似不稳定部分减少。这表明发育中的5S至16S RNA比营养细胞5S至16S RNA（假定信使RNA）更稳定。然而，在45分钟的追赶期间，以30分钟脉冲标记的发育5S至16S RNA的放射性衰变程度是位于蔗糖密度梯度的16S和23S区域的发育RNA的两倍，并且稳定性远低于蔗糖密度梯度。在30分钟的营养细胞脉冲中标记的5S，16S和23S RNA物种。

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