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首页> 外文期刊>Journal of bacteriology >Growth of Saccharomycopsis Schi?nning Under Continuous Gassing
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Growth of Saccharomycopsis Schi?nning Under Continuous Gassing

机译：连续放气下酿酒酵母的生长

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Two strains of Saccharomycopsis guttulata, JB-1 and JB-3, isolated from stomach contents of domestic rabbits, were grown under different gas phases, and their growth rates were compared. Strain JB-1 grew exponentially at a maximal growth rate under a continuous gas phase of 15% CO2, 2% O2 in nitrogen. High cell yields with low cell granulation were obtained. The growth rates were almost the same between oxygen concentrations of 0.25 and 20% at 15% CO2. Poor growth and early cell granulation occurred in the absence of oxygen at 15% CO2. Growth increased at 2% O2 in direct proportion to the carbon dioxide concentration up to 10 to 15% CO2. A very high carbon dioxide content (e.g. 98%) was somewhat inhibitory. Cell granulation always occurred during the maximal stationary phase in media at pH 4, but was relatively slight at pH 5.6 or higher. Strain JB-3 responded to various gas phases in a similar manner except that it grew slowly in the absence of oxygen at 15% CO2 (pH 4). The effect of an optimal gas phase on the growth of strain JB-1 was examined in relation to other environmental conditions. In the presence of 15% CO2, 2% O2, this strain grew exponentially in yeast autolysate-Proteose Peptone-glucose medium at 37 C at pH 2, 4, and 5.6 at approximately the same rate; the growth rate was somewhat lower at pH 6.2. Under similar conditions, strain JB-1 grew at 30 C and pH 4 at one-sixth its maximal growth rate. Cell granulation was greatly reduced at this temperature. With adequate CO2 strain JB-1 also grew at a reduced rate in a yeast autolysate medium previously reported not to support growth. Results indicate that continuous gassing with an optimal gas phase increases the growth rate to the extent that the growth rate surpasses the death rate by a significant margin; as a result, granulated cells can be avoided almost entirely in the log phase.
机译：从家兔的胃中分离出两株 Saccharomycopsis guttulata ，分别在不同的气相下生长，并比较了它们的生长速率。在氮气中15％CO 2 ，2％O 2 的连续气相下，菌株JB-1以最大的速率成倍增长。获得了具有低细胞颗粒化的高细胞产量。在15％CO 2 下，氧气浓度在0.25和20％之间时，增长率几乎相同。在15％CO 2 不存在氧气的情况下，生长不良且细胞早粒化。在2％的O 2 处，与二氧化碳浓度高达10％到15％的CO 2 成正比的增长。很高的二氧化碳含量（例如98％）在某种程度上具有抑制作用。细胞颗粒化总是发生在培养基中最大的静止期，在 p H 4处，但在 p H 5.6或更高处相对较小。 JB-3菌株以相似的方式对各种气相作出反应，不同的是它在15％CO 2 （ p H 4）不存在氧气的情况下缓慢生长。相对于其他环境条件，研究了最佳气相对菌株JB-1的生长的影响。在存在15％CO 2 ，2％O 2 的情况下，此菌株在酵母的自溶产物-蛋白P-葡萄糖培养基中于 p < / em> H 2、4和5.6的速率大致相同;在 p H 6.2处生长速度略低。在相似的条件下，菌株JB-1在30 C和 p H 4处以其最大生长速率的六分之一生长。在此温度下细胞的成粒作用大大降低。有了足够的CO 2 菌株，JB-1在以前据报道不支持生长的酵母自溶培养基中也以降低的速率生长。结果表明，以最佳气相连续放气可将增长率提高到一定程度，即增长率大大超过死亡率。结果，在对数期几乎可以避免粒状细胞。

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