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首页> 外文期刊>Journal of bacteriology >LOCALIZATION OF ENZYMES IN THE MYCELIUM AND MICROCONIDIA OF FUSARIUM OXYSPORUM
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LOCALIZATION OF ENZYMES IN THE MYCELIUM AND MICROCONIDIA OF FUSARIUM OXYSPORUM

机译：氧化镰刀菌菌丝和微分生孢子中酶的定位

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Maruyama, Yoshiharu (Cornell University, Ithaca, N.Y.) and Martin Alexander. Localization of enzymes in the mycelium and microconidia of Fusarium oxysporum. J. Bacteriol. >84:307–312. 1962—Extracts prepared from mycelium and microconidia of Fusarium oxysporum f. cubense were fractionated into a soluble and four particulate fractions by differential centrifugation, and the distribution of several enzymes in the isolated cell constituents was examined. Succinic dehydrogenase, cytochrome oxidase, and a large amount of the reduced diphosphopyridine nucleotide (DPNH) cytochrome c reductase and reduced triphosphopyridine nucleotide cytochrome c reductase were associated with one of the particulate fractions prepared from the hyphae; fumarase and DPNH oxidase activities were largely found in the soluble and in a second particulate fraction. The highest recovery and concentration of diphosphopyridine nucleotidase was observed to be bound to a third type of hyphal granule. Aldolase, aconitase, glucose-6-phosphatase, and uricase were recovered entirely with the soluble mycelium constituents. Similar enzyme-distribution patterns were observed in microconidia. Several enzymatic activities of the mycelial extracts were compared with those in the extracts of microconidia.
机译：丸山善良（纽约州伊萨卡康奈尔大学）和马丁·亚历山大（Martin Alexander）。酶在尖孢镰刀菌菌丝体和微分生孢子中的定位。 J.细菌。 > 84： 307-312。 1962年-从尖孢镰刀菌（Fusarium oxysporum） f的菌丝体和微分生孢子制备的提取物。通过差异离心法将 cubense 分为可溶部分和四个颗粒部分，并检查了几种酶在分离的细胞成分中的分布。琥珀酸脱氢酶，细胞色素氧化酶和大量还原的二磷酸吡啶核苷酸（DPNH）细胞色素 c 还原酶和还原的三磷酸吡啶核苷酸细胞色素 c 还原酶与其中一种颗粒相关从菌丝准备;富马酸酶和DPNH氧化酶的活性主要存在于可溶性和第二颗粒级分中。观察到二磷酸吡啶核苷酸酶的最高回收率和浓度与第三种菌丝颗粒结合。醛缩酶，乌头酸酶，葡萄糖-6-磷酸酶和尿酸酶完全被可溶性菌丝体成分回收。在微分生孢子中观察到相似的酶分布模式。比较了菌丝体提取物和微分生孢子提取物中几种酶的活性。

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