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首页> 外文期刊>Journal of bacteriology >Mutants of Salmonella anatum that block bacteriophage epsilon infection at early stages.
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Mutants of Salmonella anatum that block bacteriophage epsilon infection at early stages.

机译：沙门氏菌突变体可在早期阶段阻止噬菌体ε感染。

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A total of six mutants, to which phage epsilon could adsorb but failed to lyse, were isolated from Salmonella anatum and characterized. A significant portion of active phage particles could be recovered from the phage-bacterium complexes before they became irreversibly absorbed, and adsorbed phage did not kill these mutants at all. These reversibly adsorbed phage had become sensitive to chloroform, at least in some cases. The results indicate that the steps that may be blocked are deoxyribonucleic acid ejection or penetration. The mutants were tentatively classified into three groups by their susceptibility to phages c341 and Felix O. The inhibition of phage infection was overcome by host range mutants of phages epsilon and c341. The isolated lipopolysaccharide from the parent strain inactivated phages epsilon, c341, and a host range mutant of epsilon in vitro. However, neither phage epsilon nor c341, or the host range mutant of phage epsilon, was inactivated by incubation with the mutant lipopolysaccharides, even when they were derived from the mutants susceptible to c341 or the host range mutant of epsilon. These results may suggest that more than the receptor lipopolysaccharide of the bacterial surface is involved in the early stages of phage infection.
机译：从沙门氏菌中分离并鉴定了总共六个突变体，噬菌体ε可以吸附到该突变体上，但未能裂解。在噬菌体-细菌复合物被不可逆地吸收之前，可以从噬菌体-细菌复合物中回收很大一部分活性噬菌体颗粒，并且被吸附的噬菌体根本不能杀死这些突变体。这些可逆吸附的噬菌体至少在某些情况下对氯仿变得敏感。结果表明，可能被阻止的步骤是脱氧核糖核酸的喷射或渗透。根据其对噬菌体c341和Felix O的易感性，将该突变体初步分为三类。噬菌体epsilon和c341的宿主范围突变体克服了对噬菌体感染的抑制作用。从亲本菌株中分离出的脂多糖可在体外灭活噬菌体ε，c341和ε宿主范围突变体。然而，噬菌体ε或c341或噬菌体ε的宿主范围突变体都没有通过与突变体脂多糖一起孵育而被灭活，即使它们衍生自对c341或ε的宿主范围突变体敏感的突变体。这些结果可能表明，在噬菌体感染的早期阶段涉及的细菌表面多于受体脂多糖。

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