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首页> 外文期刊>Journal of bacteriology >Bromodeoxyuridine 5'-monophosphate incorporation into yeast nuclear and mitochondrial deoxyribonucleic acid.
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Bromodeoxyuridine 5'-monophosphate incorporation into yeast nuclear and mitochondrial deoxyribonucleic acid.

机译：溴脱氧尿苷5'-单磷酸盐掺入酵母核和线粒体脱氧核糖核酸中。

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摘要

Standard laboratory yeast strains can be enriched for thymidine 5'-monophosphate (TMP) uptake derivatives that generate only a low percentage of respiratory-deficient colonies (petites) under inhibition of TMP biosynthesis. Such mutants incorporated bromodeoxyuridine 5'-monophosphate (BrdUMP) into both nuclear and mitochondrial deoxyribonucleic acid (mtDNA); however, they showed a selectivity for TMP over BrdUMP incorporation. The preferential incorporation of [3H]TMP or BrdUMP into mtDNA was strain dependent. The density increments after growth in the presence of BrdUMP reached 50 mg/ml for nuclear DNA and 22 mg/ml for mtDNA in CsCl gradients. Density shifts corresponding to 4% bromouracil substitution were easily detected. Preliminary density transfer experiments confirm that mtDNA does not replicate in synchrony with nuclear DNA.
机译：标准实验室酵母菌株可富集胸腺嘧啶5'-单磷酸（TMP）摄取衍生物，在TMP生物合成受抑制的情况下，这些衍生物仅产生低百分比的呼吸缺陷菌落（娇小）。这样的突变体将溴脱氧尿苷5'-单磷酸（BrdUMP）掺入核和线粒体脱氧核糖核酸（mtDNA）;但是，它们显示出对BrdUMP掺入的TMP有选择性。 [3H] TMP或BrdUMP优先掺入mtDNA取决于菌株。在CsCl梯度中，在存在BrdUMP的情况下生长后，核DNA的密度增加达到50 mg / ml，mtDNA的密度增加达到22 mg / ml。容易检测到对应于4％溴尿嘧啶取代的密度变化。初步的密度转移实验证实，mtDNA不会与核DNA同步复制。

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