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首页> 外文期刊>Journal of bacteriology >Genetic Exchange Between Escherichia coli Strains in the Mouse Intestine
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Genetic Exchange Between Escherichia coli Strains in the Mouse Intestine

机译：小鼠肠道中大肠杆菌菌株之间的遗传交换

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Germ-free mice contaminated with selected Escherichia coli strains were used for experiments designed to demonstrate gene transfer and recombinant formation in vivo. The well-characterized conjugation system of E. coli K-12 was examined in these experiments. Contamination of germ-free mice with a polyauxotrophic F? strain followed by the addition of isogenic Hfr, F′, or F+ strains resulted in the appearance of all recombinant classes at frequencies that would be expected from an in vitro mating experiment. Inheritance of unselected donor markers occurred at frequencies that were dependent on linkage relationships established in experiments in vitro. The presence of Lactobacillus had no influence on gene transfer and recombinant formation in an F′ × F? in vivo mating. The R factor ROR-1 was transferred from E. coli strain M7-18 to an E. coli F? strain in the mouse intestine.
机译：使用被选定的大肠杆菌菌株污染的无毒小鼠进行实验，以证明其在体内的基因转移和重组形成。充分表征的 E共轭系统。在这些实验中检查了大肠杆菌 K-12。用营养缺陷型F ？菌株污染无菌小鼠，然后添加同基因的Hfr，F'或F + 菌株导致所有重组类均出现在体外交配实验所期望的频率。未选择的供体标记的遗传发生的频率取决于体外实验中建立的连锁关系。乳杆菌的存在对F'×F ？体内交配的基因转移和重组形成没有影响。 R因子ROR-1从 E转移。大肠杆菌菌株M7-18转化为 E。小鼠肠道中的大肠杆菌 F ？菌株。

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