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首页> 外文期刊>Journal of bacteriology >Parameters governing bacterial regeneration and genetic recombination after fusion of Bacillus subtilis protoplasts.
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Parameters governing bacterial regeneration and genetic recombination after fusion of Bacillus subtilis protoplasts.

机译：枯草芽孢杆菌原生质体融合后控制细菌再生和基因重组的参数。

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摘要

Bacterial protoplast fusion, induced by polyethylene glycol, has been made more regular and convenient by further specification and improvement of various steps in the previously used procedure. These have made it possible to obtain regularly 100% regeneration of Bacillus subtilis cells from protoplasts before treatment with polyethylene glycol and yields of 10 to 75% from polyethylene glycol-treated protoplasts. Genetic recombination frequencies do not increase correspondingly. Also, when regeneration is reduced by various experimental conditions, recombination does not decrease in proportion. It is concluded that regeneration of recombinant-forming cells is independently determined and not closely related to the average regeneration for the population. Kinetic studies with varying individual parental or total protoplast concentrations strongly indicate that protoplast collision and contact is not the limiting factor determining the number of genetic recombinants obtained. Recombination approximates a linear, rather than quadratic, function of the total or of the majority protoplast population present, from which it is concluded that fusion events are always adequate to produce substantially more potential recombinants than are registered. The strong effect of the majority/minority ratio upon the number of minority cells that become recombinant is independent of which parent is in excess. This shows in a direct and physiological way that both parents are equivalent partners in their genetic contributions.
机译：通过进一步规范和改进先前使用的程序中的各个步骤，已使由聚乙二醇诱导的细菌原生质体融合更加规则和方便。这些使得有可能在用聚乙二醇处理之前从原生质体中定期获得枯草芽孢杆菌细胞100％的再生，并从经聚乙二醇处理的原生质体中获得10％至75％的产率。遗传重组频率不会相应增加。另外，当通过各种实验条件减少再生时，重组不会按比例减少。结论是重组形成细胞的再生是独立确定的，与群体的平均再生没有密切关系。用不同的亲本或总原生质体浓度变化进行的动力学研究强烈表明，原生质体的碰撞和接触不是决定获得的基因重组体数量的限制因素。重组接近存在的全部或大多数原生质体群体的线性函数，而不是二次函数，由此可以得出结论，融合事件总是足以产生比所记录的实质上更多的潜在重组体。多数/少数比例对变为重组的少数细胞数量的强烈影响与哪个亲本过量无关。这以直接和生理的方式表明，父母双方在遗传贡献上是同等的伴侣。

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