...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Genetic Recombination in Bacillus subtilis 168: Effects of recU and recSMutations on DNA Repair and Homologous Recombination
【24h】

Genetic Recombination in Bacillus subtilis 168: Effects of recU and recSMutations on DNA Repair and Homologous Recombination

机译：枯草芽孢杆菌168中的基因重组：recU和recSMutation对DNA修复和同源重组的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus subtilis recombination-deficient mutants were constructed by inserting a selectable marker (cat gene) into the yppB and ypbC coding regions. TheyppB:cat and ypbC:catnull alleles rendered cells sensitive to DNA-damaging agents, impaired plasmid transformation (25- and 100-fold), and moderately affected chromosomal transformation when present in an otherwise Rec+ B. subtilis strain. The yppBgene complemented the defect of the recG40 strain.yppB and ypbC and their respective null alleles were termed “recU” and “recU1” (recU:cat) and “recS” and “recS1” (recS:cat), respectively. The recU and recS mutations were introduced into rec-deficient strains representative of the α (recF), β (addA5 addB72), γ (recH342), and ? (recG40) epistatic groups. The recU mutation did not modify the sensitivity ofrecH cells to DNA-damaging agents, but it did affect inter- and intramolecular recombination in recH cells. TherecS mutation did not modify the sensitivity ofaddAB cells to DNA-damaging agents, and it marginally affected recF, recH, and recUcells. The recS mutation markedly reduced (about 250-fold) intermolecular recombination in recH cells, and there were reductions of 10- to 20-fold in recF, addAB, and recU cells. Intramolecular recombination was blocked inrecS recF, recS addAB, and recS recU cells. RecU and RecS have no functional counterparts inEscherichia coli. Altogether, these data indicate that therecU and recS proteins are required for DNA repair and intramolecular recombination and that the recF(α epistatic group), addAB (β), recH (γ),recU (?), and recS genes provide overlapping activities that compensate for the effects of single mutation. We tentatively placed recS within a new group, termed “ζ.”
机译：通过将选择标记（ cat 基因）插入 yppB 和 ypbC 中，构建枯草芽孢杆菌重组缺陷型突变体。编码区域。 yppB ： cat 和 ypbC ： cat 无效等位基因使细胞对DNA破坏剂敏感，质粒转化受损（25倍和100倍），并且在其他Rec（sup）+ B中存在时，会适度影响染色体转化。枯草杆菌菌株。 yppB 基因弥补了 recG40 菌株的缺陷。 yppB 和 ypbC ，它们各自的无效等位基因称为“ recU ”和“ recU1 ”（ recU ： cat ）和“ recS ”和“ recS1 ”（ recS ： cat ）。将 recU 和 recS 突变引入代表 rec 缺陷型（α（ recF ））β（ addA5 addB72 ），γ（ recH342 ）和？ （ recG40 ）上位组。 recU 突变不会改变 recH 细胞对DNA破坏剂的敏感性，但确实会影响 recH 细胞中的分子间和分子内重组。 。 recS 突变不会改变 addAB 细胞对DNA破坏剂的敏感性，并且仅对 recF ， recH 产生轻微影响。 in>和 recU 单元格。 recS 突变在 recH 细胞中的分子间重组显着降低（约250倍），而 recF ， addAB 和 recU 单元格。 recS recF ， recS addAB 和 recS recU 细胞中的分子内重组被阻断。 RecU和RecS在大肠杆菌中没有功能对应物。总而言之，这些数据表明 recU 和 recS 蛋白是DNA修复和分子内重组所必需的，而 recF （α上位基团） addAB （β）， recH （γ）， recU （？）和 recS 基因提供重叠的活性，补偿单突变的影响。我们将 recS 暂时放置在一个称为“ζ”的新组中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号