首页> 外文期刊>Journal of bacteriology >Covalently closed circular deoxyribonucleic acids in spiroplasmas.
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Covalently closed circular deoxyribonucleic acids in spiroplasmas.

机译:螺旋体共价闭合的环状脱氧核糖核酸。

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Ten of twelve spiroplasma strains from different sources carried multiple covalently closed circular duplex deoxyribonucleic acid molecules, as shown by ethidium bromide-cesium chloride gradient centrifugation of cell lysates and examination of resulting bands by electron microscopy and agarose gel electrophoresis. Two to eight size classes per strain, comprising molecules of masses from 1 X 10(6) to 26 X 10(6), were detected. Several size classes of molecules were found in common in different spiroplasma strains. The amount of covalently closed circular deoxyribonucleic acid per strain was as much as 12% of total cellular deoxyribonucleic acid. The presence of sizes of the circular molecules appeared unrelated to either carriage or active production of known spiroplasma viruses, and it is tentatively concluded that they are plasmids rather than genomes or replicative forms of viruses.
机译:不同来源的十二种螺旋体菌株中的十种携带多个共价闭合的环状双链体脱氧核糖核酸分子,如溴化乙锭-氯化铯梯度裂解细胞裂解液并通过电子显微镜和琼脂糖凝胶电泳检查所得条带所示。每个菌株检测到2到8个大小级别,包括质量分子从1 X 10(6)到26 X 10(6)的分子。在不同的螺旋体菌株中发现了几种大小大小的分子。每个菌株的共价闭合的环状脱氧核糖核酸的量高达总细胞脱氧核糖核酸的12%。圆形分子大小的出现似乎与已知螺旋体病毒的携带或主动产生无关,并初步得出结论,它们是质粒而不是病毒的基因组或复制形式。

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