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首页> 外文期刊>Journal of bacteriology >Three deoxyribonucleic acid-dependent adenosine triphosphatases from Bacillus subtilis.
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Three deoxyribonucleic acid-dependent adenosine triphosphatases from Bacillus subtilis.

机译：来自枯草芽孢杆菌的三种脱氧核糖核酸依赖性腺苷三磷酸酶。

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摘要

We have isolated from Bacillus subtilis three deoxyribonucleic acid (DNA)-dependent adenosine triphosphatases (ATPases) (gamma-phosphohydrolases). The enzymes were extensively purified, and their physicochemical and functional properties were determined. The three enzymes (ATPases I, II, and III) were shown to be different by several criteria. ATPases II and III showed an absolute requirement for single-stranded DNA as a cofactor, whereas ATPase I had some residual activity also with double-stranded DNA. They required Mg2+ and had a pH optimum of 6.5 to 7. Only adenosine 5'-triphosphate and deoxyadenosine 5'-triphosphate were hydrolyzed. The molecular weights of ATPases I, II, and III were 108,000, 115,000, and 148,000, respectively. Km values for adenosine 5'-triphosphate and DNA were also evaluated and shown to be different for each enzyme. All three enzymes formed physical complexes with single-stranded DNA. We present evidence that ATPases I and II might migrate along DNA during adenosine 5'-triphosphate hydrolysis. On the other hand, this effect was not observed with ATPase III, which exhibited the highest affinity for single-stranded DNA.
机译：我们从枯草芽孢杆菌中分离了三种脱氧核糖核酸（DNA）依赖性腺苷三磷酸酶（ATPase）（γ-磷酸水解酶）。酶被广泛纯化，并确定其理化和功能特性。三种标准显示三种酶（ATPases I，II和III）不同。 ATPases II和III显示出对单链DNA作为辅因子的绝对要求，而ATPase I对双链DNA也具有一些残留活性。他们需要Mg2 +，最适pH值为6.5至7。只有腺苷5'-三磷酸和脱氧腺苷5'-三磷酸被水解。 ATPase I，II和III的分子量分别为108,000、115,000和148,000。还评估了5'-三磷酸腺苷和DNA的Km值，发现每种酶的Km值不同。这三种酶都与单链DNA形成了物理复合物。我们提供的证据表明，在5'-三磷酸腺苷水解过程中，ATPase I和II可能会沿着DNA迁移。另一方面，ATPase III没有观察到这种作用，ATPase III对单链DNA的亲和力最高。

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