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首页> 外文期刊>Journal of bacteriology >Role of inositol-containing sphingolipids in Saccharomyces cerevisiae during inositol starvation.
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Role of inositol-containing sphingolipids in Saccharomyces cerevisiae during inositol starvation.

机译：肌醇饥饿期间，含肌醇的鞘脂在啤酒酵母中的作用。

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The in vitro lipid requirements of UDP-N-acetylglucosamine-dolichol phosphate N-acetylglucosamine-1-phosphotransferase for the inositol-containing sphingolipids from Saccharomyces cerevisiae were characterized in terms of concentration and specificity. The effects of combinations of lipids, especially phosphatidylinositol and the inositol-containing sphingolipids, were also tested on the transferase. Phosphatidylinositol and phosphatidylglycerol stimulated the enzyme 3.3- and 2.8-fold, respectively. The inositol-containing sphingolipids, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine did not stimulate the activity of the transferase. Phosphatidylcholine and phosphatidylethanolamine in combination with phosphatidylinositol had no effect on the transferase activity; however, the inositol-containing sphingolipids markedly inhibited the stimulation of the transferase by phosphatidylinositol. This inhibition by the sphingolipids was prevented if phosphatidylcholine, in addition to the other lipids, was present in the assay mixture. In addition, changes due to inositol starvation in the in vivo membrane lipid environment, i.e., phosphatidylinositol and the inositol-containing sphingolipids, were analyzed to determine whether they corresponded to the observed in vitro effects. Three hours after the beginning of inositol starvation, there were 9- and 14-fold reductions in the accumulation of phosphatidylinositol in membrane fractions IIA (vesicles) and IV (endoplasmic reticulum), respectively, although there was only a 6-fold reduction in membrane fraction I (plasma membrane). The accumulation of [14C]inositol into inositol-containing sphingolipids also reflected the differences in the cellular location of membranes.
机译：从浓度和特异性方面表征了UDP-N-乙酰氨基葡糖-二羟磷酸磷酸N-乙酰氨基葡糖-1-磷酸转移酶对来自酿酒酵母的含肌醇的鞘脂的体外脂质需求。还对转移酶测试了脂质，特别是磷脂酰肌醇和含肌醇的鞘脂的组合的作用。磷脂酰肌醇和磷脂酰甘油分别刺激了该酶3.3倍和2.8倍。含肌醇的鞘脂，磷脂酰胆碱，磷脂酰乙醇胺和磷脂酰丝氨酸不会刺激转移酶的活性。磷脂酰胆碱和磷脂酰乙醇胺与磷脂酰肌醇的结合对转移酶活性没有影响。然而，含肌醇的鞘脂显着抑制磷脂酰肌醇对转移酶的刺激。如果测定混合物中除了其他脂质外还存在磷脂酰胆碱，则可以防止鞘脂的这种抑制作用。另外，分析了在体内膜脂质环境中因肌醇饥饿引起的变化，即磷脂酰肌醇和含肌醇的鞘脂，以确定它们是否对应于观察到的体外作用。肌醇饥饿开始三小时后，膜部分IIA（囊泡）和IV（内质网）中磷脂酰肌醇的积聚分别减少了9倍和14倍，尽管膜仅减少了6倍I部分（质膜）。 [14 C]肌醇积累到含肌醇的鞘脂中也反映了细胞膜位置的差异。

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