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首页> 外文期刊>Journal of bacteriology >Enzymatic deacylation of lipoteichoic acid by protoplasts of Streptococcus faecium (Streptococcus faecalis ATCC 9790).
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Enzymatic deacylation of lipoteichoic acid by protoplasts of Streptococcus faecium (Streptococcus faecalis ATCC 9790).

机译:粪链球菌(Streptococcus faecalis ATCC 9790)原生质体对脂磷壁酸的酶促脱酰作用。

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摘要

High-molecular-weight, micellar lipoteichoic acid (LTA) was converted to a lower-molecular-weight, apparently deacylated polymer when the former was incubated in the presence of growing protoplasts of Streptococcus faecium (S. faecalis ATCC 9790), but not when incubated in fresh or spent protoplast medium. The mobility of the low-molecular-weight polymer upon agarose gel electrophoresis was indistinguishable from that of native extracellular lipoteichoic acid LTA(X) from this organism or from chemically deacylated LTA. Native LTA(X) was shown to contain less than one fatty acid equivalent per 18 LTA(X) molecules, in contrast to the 4:1 ratio of fatty acids to polyglycerolphosphate chains in micellar LTA.
机译:在粪便链球菌(S. faecalis ATCC 9790)的原生质体存在下孵育前,将高分子量胶束脂蛋白酸(LTA)转化为低分子量,明显脱酰基的聚合物,但当在新鲜或用过的原生质体培养基中孵育。琼脂糖凝胶电泳后低分子量聚合物的迁移率与该生物体或化学脱酰LTA的天然细胞外脂蛋白酸LTA(X)的迁移率没有区别。相对于胶束LTA中脂肪酸与聚甘油磷酸酯链的比例为4:1,天然LTA(X)每18个LTA(X)分子包含少于一个脂肪酸当量。

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