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首页> 外文期刊>Journal of bacteriology >Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b.
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Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b.

机译:通过使用L-蛋氨酸-DL-磺胺嘧啶和谷氨酰胺营养缺陷型gln-1b研究了神经孢菌中硝酸盐还原酶的抑制作用。

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The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine could repress nitrate reductase. In a mutant of Neurospora, gln-1b, which requires glutamine for growth, only glutamine could repress nitrate reductase. These results suggest a direct role for glutamine as corepressor of nitrate reductase in Neurospora.
机译:研究了谷氨酰胺合成酶抑制剂L-蛋氨酸-DL-亚磺酰亚胺对在存在铵离子和谷氨酰胺的野生型神经孢菌中硝酸还原酶形成的影响。在使谷氨酰胺合成酶失活的条件下,发现只有谷氨酰胺可以抑制硝酸还原酶。在Neurospora的gln-1b突变体(需要谷氨酰胺生长)中,只有谷氨酰胺可以抑制硝酸还原酶。这些结果表明谷氨酰胺直接作为神经孢菌中硝酸还原酶的核心抑制剂。

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