Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides.

G A Neale; A Mitchell; L R Finch

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Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides.

机译：支原体支原体亚种中嘧啶脱氧核糖核苷酸生物合成的途径。杀菌剂。

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By measuring the specific activity of deoxyribonucleotides isolated from DNA after the incorporation of 14C-labeled precursors with and without competition from other nucleotide precursors, we defined the major pathways of pyrimidine deoxyribonucleotide synthesis in Mycoplasma mycoides subsp. mycoides. Uracil, guanine, and thymine are required for the synthesis of nucleotides. Cytidine competed effectively with uracil to provide all of the deoxycytidine nucleotide, as well as most of the deoxyribose-1-phosphate, for the synthesis of thymidylate from thymine via thymidine phosphorylase. Each of dUMP, dCMP, and dTMP competed with cytidine for incorporation into DNA thymidylate. Appreciable incorporation of exogenous deoxyribonucleoside 5'-monophosphates into DNA without prior dephosphorylation was observed. Dephosphorylation also occurred since the added deoxyribonucleotide provided phosphate for the synthesis of the other nucleotides in DNA in competition with the 32Pi in the growth medium. Hydroxyurea inhibited cell growth and decreased the intracellular level of dATP, consistent with the action of a ribonucleoside diphosphate reductase with regulatory properties similar to those of the Escherichia coli enzyme.

机译：通过测量从14 C标记的前体掺入后有无其他核苷酸前体竞争的DNA中分离的脱氧核糖核苷酸的比活，我们定义了支原体支原体亚种中嘧啶脱氧核糖核苷酸合成的主要途径。杀菌剂。合成核苷酸需要尿嘧啶，鸟嘌呤和胸腺嘧啶。胞嘧啶可与尿嘧啶有效竞争，以提供所有的脱氧胞苷核苷酸以及大部分的脱氧核糖-1-磷酸，以通过胸苷磷酸化酶从胸腺嘧啶中合成胸苷。 dUMP，dCMP和dTMP中的每一种都与胞嘧啶核苷竞争，以掺入DNA胸苷酸中。观察到外源的脱氧核糖核苷5'-单磷酸可适当地掺入DNA，而无需事先进行去磷酸化。由于添加的脱氧核糖核苷酸与生长培养基中的32Pi竞争，为DNA中其他核苷酸的合成提供了磷酸盐，因此也发生了脱磷酸作用。羟基脲抑制细胞生长并降低dATP的细胞内水平，这与核糖核苷二磷酸还原酶的作用一致，其调节特性与大肠杆菌酶相似。

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《Journal of bacteriology》 |1983年第1期|共6页
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G A Neale; A Mitchell; L R Finch;
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1. Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides. [J] . G A Neale, A Mitchell, L R Finch Journal of bacteriology . 1983,第3期

机译：支原体支原体亚种中嘧啶脱氧核糖核苷酸代谢的酶。杀菌剂。
2. Uptake and utilization of deoxynucleoside 5'-monophosphates by Mycoplasma mycoides subsp. mycoides. [J] . G A Neale, A Mitchell, L R Finch Journal of bacteriology . 1984,第3期

机译：支原体支原体亚种对脱氧核苷5'-单磷酸的吸收和利用。杀菌剂。
3. Uptake and utilization of deoxynucleoside 5'-monophosphates by Mycoplasma mycoides subsp. mycoides. [J] . G A Neale, A Mitchell, L R Finch Journal of bacteriology . 1984,第3期

机译：支原体支原体亚种对脱氧核苷5'-单磷酸的吸收和利用。杀菌剂。
4. Molecular mechanisms of virulence and antigenicity of Mycoplasma mycoides subsp. mycoides SC: Conclusions for prevention and control of CBPP [C] . Joachim Frey, Paola Pilo, Edy M. Vilei Food and Agriculture Organization of the United Nations-International Epizootic Office-Australia/Brazil-International Atomic Energy Agency Consultative Group on Contagious Bovine Pleuropneumonia . 2004

机译：毒力和支原体霉菌菌毒力和抗原性的分子机制。 Mycoides SC：用于预防和控制CBPP的结论
5. Exploring the Cell Surface: Identification and Characterization of Lipoproteins in Mycoplasma mycoides subsp. mycoides Large Colony, Mycoplasma mycoides subsp. capri, and Mycoplasma capricolum subsp. capricolum [D] . Martin, Tara R. 2009

机译：探索细胞表面：支原体支原体亚种中脂蛋白的鉴定和表征。大菌落，支原体亚种。卡普里岛和支原体亚种。癸酸
6. Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides. [O] . G A Neale, A Mitchell, L R Finch 1983

机译：支原体支原体亚种中嘧啶脱氧核糖核苷酸生物合成的途径。杀菌剂。
7. Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides [O] . G A Neale, A Mitchell, L R Finch 1983

机译：嘧啶脱氧核糖核苷酸代谢在支原体中的酶。 mycoides.

Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides.

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